microRNA‐501‐5p promotes cell proliferation and migration in gastric cancer by downregulating LPAR1
| Autor: | Wenjuan Tang, Ming Lu, Qinghong Zhao, Jianbo Han, Li Yang, Jiaxi Feng, Xiang Ma, Xiagang Luo |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Down-Regulation Apoptosis Biochemistry Flow cytometry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Stomach Neoplasms microRNA Biomarkers Tumor Tumor Cells Cultured medicine Humans Gene silencing Receptors Lysophosphatidic Acid Molecular Biology Cell Proliferation medicine.diagnostic_test Cell growth Chemistry Cell Biology Cell cycle Prognosis Molecular biology Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis |
| Zdroj: | Journal of Cellular Biochemistry. 121:1911-1922 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.29426 |
| Popis: | In spite of the achievement in treatment, the gastric cancer (GC) mortality still remains high. MicroRNAs (miRNAs) are a group of small noncoding RNAs that play a crucial part in tumor progression. In this study, we explored the expression and function of microRNA-501-5p (miR-501-5p) in GC cell lines. Quantitative real-time polymerase chain reaction assay results suggested that miR-501-5p was significantly upregulated in GC tissues and cell lines. And, the Cell Counting Kit-8 colony formation and cell migration assay results showed that the downregulation of miR-501-5p decreased GC cell proliferation and migration. Besides that, we found that GC cell cycle was arrested in G2 phase and cell apoptosis rate was increased by silencing the expression of miR-501-5p in GC cell lines using the flow cytometry. We also found that miR-501-5p could directly target lysophosphatidic acid receptor 1 (LPAR1) and negatively regulate LPAR1 expression in GC cell lines by performing dual-luciferase reporter gene assay and Western blot analysis. And, LPAR1 was significantly downregulated in GC tissues and inversely correlated with miR-501-5p expression. Furthermore, LPAR1 downregulation promoted cell proliferation and migration, which were attenuated by cotransfection of miR-501-5p inhibitor in GC cells. In conclusion, miR-501-5p can promote GC cell proliferation and migration by targeting and downregulating LPAR1. miR-501-5p/LPAR1 may become a potential therapeutic target for GC treatment. |
| Databáze: | OpenAIRE |
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