Melatonin protection against lead-induced changes in human neuroblastoma cell cultures
| Autor: | Josephine Heinz, Mohan C. Vemuri, Challa Suresh, Adanna O. Dennis, Chellu S. Chetty |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Caspase 3 Apoptosis 010501 environmental sciences Biology Toxicology 030226 pharmacology & pharmacy 01 natural sciences Neuroprotection Dinoprostone Melatonin 03 medical and health sciences chemistry.chemical_compound Neuroblastoma 0302 clinical medicine Internal medicine Cell Line Tumor medicine Humans Prostaglandin E2 IC50 0105 earth and related environmental sciences Cell Proliferation Cell growth Neurotoxicity Glutathione medicine.disease Oxidative Stress Endocrinology Neuroprotective Agents chemistry Lead medicine.drug |
| Zdroj: | International journal of toxicology. 25(6) |
| ISSN: | 1091-5818 |
| Popis: | The nervous system is the primary target for low-levels of lead (Pb) exposure and the developing brain appears to be especially vulnerable to Pb neurotoxicity. Chronic low-level Pb exposure causes growth retardation and intellectual impairment. In the present study the protective effect of melatonin during exposure to low-levels of Pb in human SH-SY5Y neuroblastoma cell cultures was assessed. The cells were exposed to Pb (0.01 to 10 μM) for 48 h. Pb inhibited the proliferation of neuroblastoma cells significantly in a concentration-dependent manner. A 50% inhibition (IC50) of cell proliferation was observed at about 5 μM Pb. Pb decreased (16% to 62%) the levels of total cellular glutathione (GSH) in a concentration (0.1 to 10 μM)-dependent manner. Exposure of cells to Pb (5 μM) for 48 h resulted in an eightfold increase in caspase-3 activity and prostaglandin E2(PGE2) level. Pretreatment with melatonin (10 μM) blocked the effects of Pb on GSH content and caspase-3 activity, and showed significant improvement in reducing the level of PGE2. The results suggest that some of the neurotoxic effects of Pb may be partly mediated by apoptosis and pretreatment with melatonin can prevent these effects. The present study asserts the neuroprotective effect of melatonin in conditions of Pb-induced toxicity in neuroblastoma cell cultures. |
| Databáze: | OpenAIRE |
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