Clinical validation of the PCR-reverse dot blot human papillomavirus genotyping test in cervical lesions from Chinese women in the Fujian province: a hospital-based population study
| Autor: | Yafang Kang, Binhua Dong, Xiaodan Mao, Guanyu Ruan, Pengming Sun, Fen Lin, Yiyi Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology Pathology Biopsy Uterine Cervical Neoplasms Cervix Uteri Polymerase Chain Reaction Cervix 0302 clinical medicine Cancer screening Medicine Papillomaviridae Early Detection of Cancer Cervical cancer Colposcopy Aged 80 and over education.field_of_study medicine.diagnostic_test biology HPV infection Obstetrics and Gynecology virus diseases General Medicine Middle Aged female genital diseases and pregnancy complications Hospitals 030220 oncology & carcinogenesis Original Article Female Cancer Screening Adult medicine.medical_specialty China Histology Genotype Adolescent Population Cervical intraepithelial neoplasia Sensitivity and Specificity 03 medical and health sciences Young Adult Internal medicine Humans education Aged Vaginal Smears business.industry Papillomavirus Infections Cancer Cell Biology Sequence Analysis DNA medicine.disease biology.organism_classification Uterine Cervical Dysplasia 030104 developmental biology DNA Viral business |
| Zdroj: | Journal of Gynecologic Oncology |
| ISSN: | 2005-0399 2005-0380 |
| Popis: | OBJECTIVE To determine the clinical significance of the polymerase chain reaction (PCR)-reverse dot blot (RDB) human papillomavirus (HPV) genotyping assay in cervical cancer screening. METHODS A total of 10,442 women attending the Fujian Provincial Maternity and Children's Health Hospital were evaluated using the liquid-based cytology (thinprep cytologic test [TCT]) and the PCR-RDB HPV test. Women with HPV infection and/or abnormal cytology were referred for colposcopy and biopsy. For HPV DNA sequencing, 120 specimens were randomly selected. Pathological diagnosis was used as the gold standard. RESULTS Using the PCR-RDB HPV test, overall HPV prevalence was 20.57% (2,148/10,442) and that of high-risk (HR)-HPV infection was 18.68% (1,951/10,442). There was 99.2% concordance between HPV PCR-RDB testing and sequencing. In this studied population, the most common HR-HPV types were HPV-16, -52, -58, -18, -53, -33, and -51, rank from high to low. HPV-16, -18, -58, -59, and -33 were the top 5 prevalent genotypes in cervical cancer but HPV-16, -18, -59, -45, and -33 were the top 5 highest risk factors for cancer (odds ratio [OR]=34.964, 7.278, 6.728, 6.101, and 3.658; all p |
| Databáze: | OpenAIRE |
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