Biological characteristics of adenomatous nodules, adenomas, and hyperfunctioning nodules as defined by adenylate cyclase activity and TSH receptors
| Autor: | Colin G. Thomas, Robert L. Reddick, Shihadeh N. Nayfeh, W. Combest, H. Jordan, Robert McQuade |
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| Rok vydání: | 1984 |
| Předmět: |
Adenoma
Adult Male endocrine system medicine.medical_specialty Adolescent endocrine system diseases medicine.medical_treatment Adenylate kinase Receptors Cell Surface Cyclase chemistry.chemical_compound Internal medicine Parenchyma Sodium fluoride medicine Humans Thyroid Neoplasms Alprostadil Child Aged business.industry Prostaglandins E Thyroid Thyroidectomy Receptors Thyrotropin Middle Aged Thionucleotides medicine.disease Thyroid Diseases Stimulation Chemical Enzyme Activation Endocrinology medicine.anatomical_structure chemistry Guanosine 5'-O-(3-Thiotriphosphate) Sodium Fluoride Female Surgery Guanosine Triphosphate business Cyclase activity Adenylyl Cyclases Goiter Nodular |
| Zdroj: | World Journal of Surgery. 8:445-450 |
| ISSN: | 1432-2323 0364-2313 |
| Popis: | This study was designed to examine the functional characteristics of thyroid adenomas, adenomatous (colloid) nodules, hyperfunctioning nodules, and normal thyroid parenchyma with respect to the presence of TSH receptors, levels of adenylate cyclase, and the responsiveness of adenylate cyclase to specific stimuli, e.g., NaF (sodium fluoride), GTPγS [guanosine 5′-0-(3-thiotriphosphatate)], PGE1, and TSH. Aliquots of specimens from patients undergoing thyroidectomy [11 normal thyroids, 7 adenomas, 8 adenomatous (colloid) nodules, 5 hyperfunctioning nodules] were immersed in liquid nitrogen and stored at 90°C. Studies were performed on partially purified plasma membranes. Receptor binding of125I-TSH was quantitated and analyzed according to Scatchard. Adenylate cyclase activity was assayed in the same tissue fraction by conversion of (α-32P) ATP to cAMP. |
| Databáze: | OpenAIRE |
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