Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses
| Autor: | Katja Mohrs, Jürgen van der Bosch, Helmut L. Haas, Colin M. Fitzsimmons, Michael J. Doenhoff, Markus Mohrs, Bart Everts, Georgia Perona-Wright, Cornelis H. Hokke, Maria Yazdanbakhsh, Alwin J. van der Ham, Gabriele Schramm, Hermelijn H. Smits |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Helminth protein
Immunology Priming (immunology) Receptors Cell Surface In Vitro Techniques 03 medical and health sciences Mice 0302 clinical medicine Immune system Th2 Cells Antigen In vivo parasitic diseases Immunology and Allergy Animals Humans 030304 developmental biology Glycoproteins Ovum chemistry.chemical_classification Mice Knockout 0303 health sciences biology Brief Definitive Report Cell Differentiation Dendritic Cells Helminth Proteins Schistosoma mansoni biology.organism_classification Virology In vitro Recombinant Proteins Cell biology chemistry Antigens Helminth Culture Media Conditioned Cytokines Female Glycoprotein 030215 immunology |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Soluble egg antigens of the parasitic helminth Schistosoma mansoni (S. mansoni egg antigen [SEA]) induce strong Th2 responses both in vitro and in vivo. However, the specific molecules that prime the development of Th2 responses have not been identified. We report that omega-1, a glycoprotein which is secreted from S. mansoni eggs and present in SEA, is capable of conditioning human monocyte-derived dendritic cells in vitro to drive T helper 2 (Th2) polarization with similar characteristics as whole SEA. Furthermore, using IL-4 dual reporter mice, we show that both natural and recombinant omega-1 alone are sufficient to generate Th2 responses in vivo, even in the absence of IL-4R signaling. Finally, omega-1–depleted SEA displays an impaired capacity for Th2 priming in vitro, but not in vivo, suggesting the existence of additional factors within SEA that can compensate for the omega-1–mediated effects. Collectively, we identify omega-1, a single component of SEA, as a potent inducer of Th2 responses. |
| Databáze: | OpenAIRE |
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