Alteration of Cellular RNA Synthesis and Processing in Type 7 Adenovirus-infected KB Cells
| Autor: | W. M. Shannon, S. Halperen |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Cytoplasm
Sucrose Time Factors medicine.drug_class Antibiotics Balanced salt solution Biology Tritium Adenoviridae Cell Line Virology Lactalbumin hydrolysate Centrifugation Density Gradient Protein biosynthesis medicine Humans RNA Neoplasm Uridine Cells Cultured Cellular compartment Carbon Isotopes Carcinoma RNA Ribosomal RNA Molecular biology Culture Media RNA Ribosomal Spectrophotometry Autoradiography Mouth Neoplasms Ultracentrifugation Cell Nucleolus |
| Zdroj: | Journal of General Virology. 12:321-324 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-12-3-321 |
| Popis: | It has been reported that cellular DNA-like RNA (D-RNA) synthesis, as well as protein synthesis, is inhibited when mammalian cells are infected with adenovirus type 5 (Bello & Ginsberg, 1969). It has also been reported (Hodge & Scharff, 1968; Raskas, Thomas & Green, 1970) that adenovirus type 2 induces a marked decrease in the appearance of 18 s and 28 s ribosomal RNA species in the cytoplasm of infected cells between 10 and 19 hr after infection. The present communication provides evidence that KB cells infected with adenovirus type 7 also exhibit changed patterns of host RNA synthesis and alterations in the processing and distribution of various RNA species among the cellular compartments. KB cells were propagated as monolayer cultures in 8 oz sealed prescription bottles in a medium consisting of Hanks's balanced salt solution (HBSS) containing 0.5% lactalbumin hydrolysate (LAH) and supplemented with 10% newborn-calf serum and antibiotics. |
| Databáze: | OpenAIRE |
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