Comparison of the effects of dendrimer, micelle and silver nanoparticles on phospholipase A2 structure
| Autor: | Aleksandr Sukhodola, Maria Terehova, F. Javier de la Mata, Rafael Gómez, Volha Dzmitruk, Maksim Ionov, Gevorg Ghukasyan, Elzbieta Pedziwiatr-Werbicka, Serge Mignani, Dzmitry Shcharbin, Maria Bryszewska, Gayane Kirakosyan, Xiangyang Shi, Jean-Pierre Majoral, Viktar Abashkin |
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| Přispěvatelé: | Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Dendrimers Silver [SDV]Life Sciences [q-bio] Metal Nanoparticles Bioengineering Protein Corona 01 natural sciences Applied Microbiology and Biotechnology Micelle Silver nanoparticle 03 medical and health sciences Phospholipase A2 010608 biotechnology Dendrimer Amphiphile Animals ComputingMilieux_MISCELLANEOUS Micelles biology Chemistry Cationic polymerization General Medicine Combinatorial chemistry Phospholipases A2 030104 developmental biology biology.protein Nanomedicine Nanoparticles Cattle Biotechnology |
| Zdroj: | Journal of Biotechnology Journal of Biotechnology, Elsevier, 2021, 331, pp.48-52. ⟨10.1016/j.jbiotec.2021.03.009⟩ |
| ISSN: | 1873-4863 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2021.03.009⟩ |
| Popis: | The interaction of nanoparticles (NP) with proteins (the so-called 'protein corona') is a huge challenge in attempting to apply them in personalized nanomedicine. We have analyzed the interaction between A) two 'soft' NPs (a cationic phosphorus dendrimer of generation 3; a cationic phosphorus amphiphilic dendron of generation 2), and B) one 'hard' nanoparticle (silver NP covered with cationic carbosilane dendritic moieties); and membrane-bound protein phospholipase A2 from bovine pancreas. The hard and soft NPs have differences in the nature of their interactions with phospholipase A2. This enzyme surrounds hard AgNP, whereas dendrimer and amphiphilic dendron form aggregates/micelles with phospholipase A2. There is a difference in action of phospholipase A2 bound to the core of dendrimer, and of micelles formed from non-covalent interactions between the amphiphilic dendron. These data are important in understanding the nature of interaction between different kinds of nanoparticles and proteins. |
| Databáze: | OpenAIRE |
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