The TCR γδ Repertoire and Relative Gene Expression Characteristics of T-ALL Cases with Biclonal Malignant Vδ1 and Vδ2 T Cells
| Autor: | Yangqiu Li, Tie-zhen Ye, Shaohua Chen, Yu Ma, Grzegorz K. Przybylski, Xiuli Wu, Suming Huang, Xu Wang, Haitao Zheng, Bing Xu, Lijian Yang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Subfamily Biology Gene Rearrangement T-Lymphocyte Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Real-Time Polymerase Chain Reaction law.invention Pathogenesis Young Adult law Gene expression Genetics Humans Molecular Biology Polymerase chain reaction Inflammation & Host Response to Infection T-cell receptor Receptors Antigen T-Cell gamma-delta Cell Biology General Medicine Molecular biology Gene Expression Regulation Neoplastic Reverse transcription polymerase chain reaction Child Preschool Monoclonal Comparative genomic hybridization |
| Zdroj: | DNA and Cell Biology. 33:49-56 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2013.2199 |
| Popis: | Despite significant improvement in our understanding of T-cell acute lymphoblastic leukemia (T-ALL) biology and pathogenesis, many questions remain unanswered. In previous studies, we found a T-ALL case with two malignant T-cell clones with Vδ1Dδ2Dδ3Jδ1 and Vδ2Dδ3Jδ2 rearrangements. In this study, we further characterized T-ALL cases with two malignant clones containing Vδ1Dδ3Jδ1 and Vδ2Dδ1Jδ1 rearrangements using fine-tiling array comparative genomic hybridization, ligation-mediated polymerase chain reaction (LM-PCR), sequencing, and reverse transcription polymerase chain reaction (RT-PCR) analysis. We further analyzed the distribution and clonality of the T-cell receptor (TCR) Vγ and Vδ subfamily T cells in the two T-ALL cases by RT-PCR and GeneScan. Monoclonal Vδ1 and Vδ2 subfamilies were confirmed in both samples, the Vδ3 through Vδ7 subfamilies could not be detected in the T-ALL samples, whereas the oligoclonal Vδ8 subfamily could be identified. Based on the clinical finding that both of the T-ALL cases with two malignant T-cell clones had a poor outcome, we attempted to compare the expression pattern of genes related to T-cell activation and proliferation between cases with the malignant Vδ1 and Vδ2 T-cell clones and T-ALL cases with a mono-malignant Vα T-cell clone. We selected two T-ALL cases with VαJα rearrangements and analyzed the expression level of Notch1, TAL1, and the CARMA-BCL10-MALT-A20-NF-κB pathway genes by real-time PCR. A20 had significantly higher expression in the biclonal compared with the monoclonal T-ALL group (p=0.0354), and there was a trend toward higher expression for the other genes in the biclonal group with the exception of TAL1, although the differences were not statistically significant. In conclusion, we identified two T-ALL cases with biclonal malignant T-cell clones and described the characteristics of the biclonal T-ALL subtype and its gene expression pattern. Thus, our findings may improve the understanding of biclonal T-ALL. |
| Databáze: | OpenAIRE |
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