Gene Expression Profiling of Tuberculous Meningitis Co-infected with HIV
| Autor: | Rajesh Raju, S K Shankar, Shyam Mohan Palapetta, Akhilesh Pandey, A. Chawla, Y. Subbanayya, Ghantasala S. Sameer Kumar, H. C. Harsha, Anita Mahadevan, Arivusudar Marimuthu, Renu Goel, Y. L. Ramachandra, Pramila Tata, A. K. Venugopal, Jyoti Bajpai Dikshit, Jagadeesha Maharudraiah, T. S. K. Prasad, Parthasarthy Satishchandra, Manoj Kumar Kashyap |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cell Biology
Biology biology.organism_classification medicine.disease urologic and male genital diseases Biochemistry Tuberculous meningitis Article Computer Science Applications Gene expression profiling Mycobacterium tuberculosis Immunology medicine Immunohistochemistry Thymidine phosphorylase DNA microarray HSPA8 Molecular Biology Gene |
| Zdroj: | Journal of proteomicsbioinformatics. 5(9) |
| ISSN: | 0974-276X |
| Popis: | Tuberculous meningitis (TBM) is a fatal form of Mycobacterium tuberculosis infection of the central nervous system (CNS). The similarities in the clinical and radiological findings in TBM cases with or without HIV make the diagnosis very challenging. Identification of genes, which are differentially expressed in brain tissues of HIV positive and HIV negative TBM patients, would enable better understanding of the molecular aspects of the infection and would also serve as an initial platform to evaluate potential biomarkers. Here, we report the identification of 796 differentially regulated genes in brain tissues of TBM patients co-infected with HIV using oligonucleotide DNA microarrays. We also performed immunohistochemical validation and confirmed the abundance of four gene products-glial fibrillary acidic protein (GFAP), serpin peptidase inhibitor, clade A member 3 (SERPINA3), thymidine phosphorylase (TYMP/ECGF1) and heat shock 70 kDa protein 8 (HSPA8). Our study paves the way for understanding the mechanism of TBM in HIV positive patients and for further validation of potential disease biomarkers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|