| Autor: |
Begam, Morium, Narayan, Neha, Mankowski, Drew, Camaj, Robert, Murphy, Nicholas, Roseni, Kevin, Pepin, Marie E., Blackmer, Jacob M., Jones, Takako I., Roche, Joseph A. |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
J Vis Exp |
| ISSN: |
1940-087X |
| Popis: |
Progressive resistance training (PRT), which involves performing muscle contractions against progressively greater external loads, can increase muscle mass and strength in healthy individuals and in patient populations. There is a need for precision rehabilitation tools to test the safety and effectiveness of PRT to maintain and/or restore muscle mass and strength in preclinical studies on small and large animal models. The PRT methodology and device described in this article can be used to perform dosage-adjusted resistance training (DART). The DART device can be used as a standalone dynamometer to objectively assess the concentric contractile torque generated by the ankle dorsiflexors in mice or can be added to a pre-existing isokinetic dynamometry system. The DART device can be fabricated with a standard 3D printer based on the instructions and open-source 3D print files provided in this work. The article also describes the workflow for a study to compare contraction-induced muscle damage caused by a single bout of DART to muscle damage caused by a comparable bout of isometric contractions (ISOM) in a mouse model of limb-girdle muscular dystrophy type 2B/R2 (BLAJ mice). The data from eight BLAJ mice (four animals for each condition) suggest that less than 10% of the tibialis anterior (TA) muscle was damaged from a single bout of DART or ISOM, with DART being less damaging than ISOM. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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