Clonal growth of bladder cancer cells in a double layer soft agar assay: needles addition of multiple culture supplements
| Autor: | G. H. Weiss, P. Smith, Kan-ei Lee, Abraham T.K. Cockett |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Bladder cancer
Urology Soft Agar Assay Tumor cells In Vitro Techniques Biology Human cell medicine.disease Tryptic soy broth Culture Media Microbiology Colony-Forming Units Assay Mice chemistry.chemical_compound Urinary Bladder Neoplasms chemistry Cell culture Tumor Cells Cultured medicine Animals Humans Tumor Stem Cell Assay Clonal growth |
| Zdroj: | Urological Research. 18:197-200 |
| ISSN: | 1434-0879 0300-5623 |
| Popis: | The necessity of multiple culture supplements, characteristic of Hamburger and Salmon (H&S) soft agar assay (1977), for the clonal growth of bladder cancer cells was systematically investigated. One murine and 6 human cell lines were cultivated in different culture supplemented conditions based on the H&S method. All cell lines successfully formed colonies in the absence of supplements. The inclusion of 2-mercaptoethanol to supplement-free medium stimulated the clonal growth of murine (MBT-2) cells, whereas it did not affect the growth of human cell lines. DEAE-dextran suppressed the clonal growth of 5 out of 7 lines. Tryptic soy broth stimulated the growth of 2 human cell lines, whereas it suppressed the other 2 cell lines. The chemosensitivity of MBT-2 cells was not affected by these supplements. |
| Databáze: | OpenAIRE |
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