Differential patterns of dehydroabietic acid biotransformation by Nicotiana tabacum and Catharanthus roseus cells
| Autor: | Petri Lackman, Hannu Maaheimo, Heli Nygren, Suvi T. Häkkinen, Kirsi-Marja Oksman-Caldentey, Heiko Rischer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Glycosylation Magnetic Resonance Spectroscopy Catharanthus Bioconversion Nicotiana tabacum Bioengineering 01 natural sciences 7. Clean energy Applied Microbiology and Biotechnology Catalysis 03 medical and health sciences Bioreactors Cytochrome P-450 Enzyme System Species Specificity Biotransformation Tobacco 030304 developmental biology Indole test 0303 health sciences Catharanthus roseus biology Hairy roots food and beverages General Medicine Metabolism 15. Life on land Cell cultures biology.organism_classification Secologanin Tryptamine Alkaloids Dehydroabietic acid Terpenoid Culture Media Kinetics Biochemistry Abietanes Resin acid 010606 plant biology & botany Biotechnology |
| Zdroj: | Journal of Biotechnology Häkkinen, S T, Lackman, P, Nygren, H, Oksman-Caldentey, K-M, Maaheimo, H & Rischer, H 2012, ' Differential patterns of dehydroabietic acid biotransformation by Nicotiana tabacum and Catharanthus roseus cells ', Journal of Biotechnology, vol. 157, no. 2, pp. 287-294 . https://doi.org/10.1016/j.jbiotec.2011.11.008 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2011.11.008 |
| Popis: | The aim of this study was to use whole cell catalysts as tools for modification of selected resin acids in order to obtain value-added functional derivatives. The enzymatic bioconversion capacities of two plant species were tested towards dehydroabietic acid. Dehydroabietic acid (DHA) is an abundant resin acid in conifers, representing a natural wood protectant. It is also one of the constituents found in by-products of the kraft chemical pulping industry. DHA was fed to tobacco (Nicotiana tabacum) and Madagascar periwinkle (Catharanthus roseus) plant cell and tissue cultures and bioconversion product formation was monitored using NMR analysis. Both plant species took up DHA from culture medium, and various types of typical detoxification processes occurred in both cultures. In addition, diverse responses to DHA treatment were observed, including differences in uptake kinetics, chemical modification of added substrate and changes in overall metabolism of the cells. Interestingly, Catharanthus roseus, a host species for pharmaceutically valuable terpenoid indole alkaloids, exhibited a very different bioconversion pattern for exogenously applied DHA than tobacco, which does not possess a terpenoid indole pathway. In tobacco, DHA is readily glycosylated in the carbonyl group, whereas in periwinkle it is proposed that a cytochrome P450-catalyzed enzymatic detoxification reaction takes place before the formation of glycosylated product. |
| Databáze: | OpenAIRE |
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