Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization
| Autor: | Lorenzo Breschi, J. H. Bian, Y. X. Ma, Ji-hua Chen, Shu Zhang, Liguo Wang, Chen yu Wang, Franklin Chi Meng Tay, Li Na Niu, Lige Tonggu, Kai Jiao, Yaodong Yang, D. X. Hao, Q. Song, Dwayne Arola, Lei Zhang |
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| Přispěvatelé: | Song Q., Jiao K., Tonggu L., Wang L.G., Zhang S.L., Yang Y.D., Zhang L., Bian J.H., Hao D.X., Wang C.Y., Ma Y.X., Arola D.D., Breschi L., Chen J.H., Tay F.R., Niu L.N. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Mineralized tissues
Models Molecular PROTEINS Materials Science TURKEY TENDON I COLLAGEN Molecular Dynamics Simulation Ligands Mineralization (biology) Apatite Collagen fibril MECHANISMS Ectopic calcification Calcification Physiologic Biomimetic Materials Biomimetics DEFORMATION medicine OSTEOPONTIN PHOSPHATE Humans Research Articles Minerals Multidisciplinary Tissue Scaffolds Ligand Chemistry SciAdv r-articles Life Sciences Mesenchymal Stem Cells medicine.disease Polyelectrolytes Polyelectrolyte Extracellular Matrix Microscopy Electron visual_art Biophysics visual_art.visual_art_medium Collagen Intrafibrillar mineralization BONE MATRICES Research Article NUCLEATION |
| Zdroj: | Science Advances |
| Popis: | Collagen-bound nucleation inhibitor ameliorates mineralization via caching of prenucleation clusters. Contemporary models of intrafibrillar mineralization mechanisms are established using collagen fibrils as templates without considering the contribution from collagen-bound apatite nucleation inhibitors. However, collagen matrices destined for mineralization in vertebrates contain bound matrix proteins for intrafibrillar mineralization. Negatively charged, high–molecular weight polycarboxylic acid is cross-linked to reconstituted collagen to create a model for examining the contribution of collagen-ligand interaction to intrafibrillar mineralization. Cryogenic electron microscopy and molecular dynamics simulation show that, after cross-linking to collagen, the bound polyelectrolyte caches prenucleation cluster singlets into chain-like aggregates along the fibrillar surface to increase the pool of mineralization precursors available for intrafibrillar mineralization. Higher-quality mineralized scaffolds with better biomechanical properties are achieved compared with mineralization of unmodified scaffolds in polyelectrolyte-stabilized mineralization solution. Collagen-ligand interaction provides insights on the genesis of heterogeneously mineralized tissues and the potential causes of ectopic calcification in nonmineralized body tissues. |
| Databáze: | OpenAIRE |
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