Enzymatic Spermine Metabolites Induce Apoptosis Associated with Increase of p53, caspase-3 and miR-34a in Both Neuroblastoma Cells, SJNKP and the N-Myc-Amplified Form IMR5
| Autor: | Pasquale Fino, Gabriella Schiera, Gianluca Canettieri, Yuta Kanamori, Italia Di Liegro, Enzo Agostinelli, Patrizia Proia, Jessica Gasparello, Roberto Gambari, Alessia Finotti, Laura Di Magno, Carlo Maria Di Liegro, Paola Tirassa, Fabio Timeus, Tomoaki Tahara, Antonio Greco |
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| Přispěvatelé: | Kanamori Y., Finotti A., Di Magno L., Canettieri G., Tahara T., Timeus F., Greco A., Tirassa P., Gasparello J., Fino P., Di Liegro C.M., Proia P., Schiera G., Di Liegro I., Gambari R., Agostinelli E. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Polyamine neuroblastoma apoptosis microRNA mitochondria reactive oxygen species oncotherapy chemistry.chemical_compound 0302 clinical medicine Annexin polyamine Settore BIO/10 - Biochimica Antineoplastic Combined Chemotherapy Protocols Cytotoxic T cell Settore BIO/06 - Anatomia Comparata E Citologia Biology (General) Membrane Potential Mitochondrial N-Myc Proto-Oncogene Protein Chemistry Caspase 3 General Medicine Blot Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Amine Oxidase (Copper-Containing) Signal Transduction QH301-705.5 Article NO 03 medical and health sciences Neuroblastoma Cell Line Tumor medicine Animals Humans Propidium iodide Rats Wistar Cell Proliferation Oncogene Gene Amplification medicine.disease Molecular biology MicroRNAs 030104 developmental biology Apoptosis Spermine Tumor Suppressor Protein p53 |
| Zdroj: | Cells, Vol 10, Iss 1950, p 1950 (2021) Cells Volume 10 Issue 8 |
| Popis: | Neuroblastoma (NB) is a common malignant solid tumor in children and accounts for 15% of childhood cancer mortality. Amplification of the N-Myc oncogene is a well-established poor prognostic marker in NB patients and strongly correlates with higher tumor aggression and resistance to treatment. New therapies for patients with N-Myc-amplified NB need to be developed. After treating NB cells with BSAO/SPM, the detection of apoptosis was determined after annexin V-FITC labeling and DNA staining with propidium iodide. The mitochondrial membrane potential activity was checked, labeling cells with the probe JC-1 dye. We analyzed, by real-time RT-PCR, the transcript of genes involved in the apoptotic process, to determine possible down- or upregulation of mRNAs after the treatment on SJNKP and the N-Myc-amplified IMR5 cell lines with BSAO/SPM. The experiments were carried out considering the proapoptotic genes Tp53 and caspase-3. After treatment with BSAO/SPM, both cell lines displayed increased mRNA levels for all these proapoptotic genes. Western blotting analysis with PARP and caspase-3 antibody support that BSAO/SPM treatment induces high levels of apoptosis in cells. The major conclusion is that BSAO/SPM treatment leads to antiproliferative and cytotoxic activity of both NB cell lines, associated with activation of apoptosis. |
| Databáze: | OpenAIRE |
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