Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification
| Autor: | Curtis J. Omiecinski, M R Andersen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Restriction Mapping medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology law.invention Microbiology Foodborne Diseases chemistry.chemical_compound Restriction map Plasmid law Escherichia coli medicine Humans Food microbiology Enteroinvasive Escherichia coli Escherichia coli Infections Polymerase chain reaction Polymerase Ecology biology Biochemistry chemistry Evaluation Studies as Topic Food Microbiology biology.protein DNA Plasmids Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 58:4080-4082 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.58.12.4080-4082.1992 |
| Popis: | In this report we describe a simple and rapid technique using DNA affinity columns that permits direct extraction of bacterial plasmids from a variety of foods for polymerase chain reaction amplification. The procedure was used to detect virulent enteroinvasive Escherichia coli in several artificially seeded matrices, including seafoods, greens, dairy products, enrichment media, and water. Polymerase inhibitors present in both foods and enrichment media were removed efficiently. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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