A ratiometric fluorogenic nanoprobe for real-time quantitative monitoring of lysosomal pH
| Autor: | Caroline Brommesson, Anna du Rietz, Zhangjun Hu, Xin Zhang, Xiongyu Wu, Kajsa Uvdal, Jiwen Hu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fluorophore
Nanoprobe 02 engineering and technology 010402 general chemistry 01 natural sciences Silica nanoparticles chemistry.chemical_compound Lysosome Materials Chemistry medicine Electrical and Electronic Engineering Instrumentation Calcium signaling Chemistry Metals and Alloys Biochemistry and Molecular Biology 021001 nanoscience & nanotechnology Condensed Matter Physics Biocompatible material 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials FRET Ratiometric fluorescent nanoprobe Lysosome targeting Bioimaging pH sensitive Förster resonance energy transfer medicine.anatomical_structure Rhodamine derivative Biophysics 0210 nano-technology Biokemi och molekylärbiologi |
| Popis: | Lysosomes are known as key players in cellular signalling and act as terminal degradation stations involved in a multitude of cellular processes. Being a highly influential physiological factor, pH is essential in the regulation of lysosome-mediated physiological and pathological processes. Aberrant pH fluctuations are highly related to lysosomal dysfunction that correlates to lysosomal storage diseases and neurodegenerative disorders. As such, real-time quantitative monitoring of lysosomal pH (pHL) is crucial for gaining insight into lysosomal dysfunction but challenging by the lack of effective lysosome-specific probes with high signal fidelity. Toward this end, we have proposed a lysosomal fluorogenic nanoprobe (TR-MP) for reliable ratiometric measuring of pHL. It is fabricated by rational manipulation of fluorescence resonance energy transfer (FRET) in a tailorable nanoplatform. The nanoprobe consists of biocompatible silica nanoparticles assembled with a pH-sensitive rhodamine derivative (RDM-TEOS) as an acceptor and aggregation-induced emission (AIE) fluorophore (TPE-OMe) as a donor to ensure high energy transfer efficiency. Further equipped with cell-penetrating facilitator and morpholine to enable effective cell-internalization and high lysosome affinity of TR-MP. Results show that TR-MP can quantitatively measure pH in a range of 3.0 - 7.0 and detect pHL fluctuations in live cells under various stimuli, as well as real-time monitor pHL during apoptosis. Funding Agencies|STINT Joint China-Sweden Mobility Project [CH2017-7243]; Swedish Research Council (VR)Swedish Research Council [VR 2019-02409, 2020-05437]; China Scholarship Council (CSC)China Scholarship Council; Carl Tryggers Stiftelse [CTS 19:379]; Swedish Government strategic faculty grant in material science (SFO, MATLIU) in Advanced Functional Materials (AFM) (VR) [5.1-2015-5959]; Centre in Nano Science and technology at LiTH (CeNano); LiU Cancer network at Linkoping University |
| Databáze: | OpenAIRE |
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