| Autor: |
James R. Byrnes, Amy M. Weeks, Eric Shifrut, Julia Carnevale, Lisa Kirkemo, Alan Ashworth, Alexander Marson, James A. Wells |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Molecular & Cellular Proteomics. 21:100217 |
| ISSN: |
1535-9476 |
| Popis: |
Immunosuppressive factors in the tumor microenvironment (TME) impair T cell function and limit the antitumor immune response. T cell surface receptors and surface proteins that influence interactions and function in the TME are proven targets for cancer immunotherapy. However, how the entire surface proteome remodels in primary human T cells in response to specific suppressive factors in the TME remains to be broadly and systematically characterized. Here, using a reductionist cell culture approach with primary human T cells and stable isotopic labeling with amino acids in cell culture-based quantitative cell surface capture glycoproteomics, we examined how two immunosuppressive TME factors, regulatory T cells (Tregs) and hypoxia, globally affect the activated CD8 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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