Ultrasensitive Determination of Sugar Phosphates in Trace Samples by Stable Isotope Chemical Labeling Combined with RPLC–MS

Autor:	Sha Li, Fei-Long Liu, Zheng Zhang, Xiao-Ming Yin, Tian-Tian Ye, Bi-Feng Yuan, Yu-Qi Feng
Rok vydání:	2022
Předmět:	Glucose Isotopes Isotope Labeling Sugar Phosphates Chromatography Liquid Phosphates Analytical Chemistry
Zdroj:	Analytical Chemistry. 94:4866-4873
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.2c00346
Popis:	Sugar phosphates are important metabolic intermediates in organisms and play a vital role in energy and central carbon metabolism. Profiling of sugar phosphates is of great significance but full of challenges due to their high structural similarity and low sensitivities in liquid chromatography (LC)-mass spectrometry (MS). In this study, we developed a novel stable isotope chemical labeling combined with the reversed-phase (RP)LC-MS method for ultrasensitive determination of sugar phosphates at the single-cell level. By chemical derivatization with 2-(diazo-methyl)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91190785718fd9fcccb7949955ea6e0a https://doi.org/10.1021/acs.analchem.2c00346 Zobrazit plný text záznamu