Determination of 8 biogenic amines in aquatic products and their derived products by high-performance liquid chromatography-tandem mass spectrometry without derivatization
| Autor: | Changling Fang, Xuan Zhang, Yunyu Tang, Cong Kong, Guangxin Yang, Youqiong Cai, Dongmei Huang, Pei Cao, Yongfu Shi |
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| Rok vydání: | 2021 |
| Předmět: |
Tryptamine
Biogenic Amines Formic acid Spermidine Tyramine Tandem mass spectrometry 01 natural sciences High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Cadaverine Phenethylamines Putrescine Perchloric acid Derivatization Chromatography High Pressure Liquid Chromatography Elution 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine 040401 food science Tryptamines 0104 chemical sciences chemistry Seafood Spermine Food Science Histamine |
| Zdroj: | Food chemistry. 361 |
| ISSN: | 1873-7072 |
| Popis: | A method for the determination of 8 biogenic amines in aquatic products and their derived products was established by HPLC-MS/MS without derivatization. The samples were extracted by 5% perchloric acid solution. N-hexane was used to clean the extract. The analytes were separated by a column of ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm), and gradient eluted with a mixed solution of (0.5% formic acid) and acetonitrile. Good linearity was obtained with correlation coefficients (R2) >0.99. This method achieved higher sensitivity (from 0.1 mg/kg for tyramine, 2-phenylethylamine and tryptamine to 1.0 mg/kg for spermidine, spermine, cadaverin, histamine and putrescine). The average recoveries were demonstrated in the range of 70.9%–113.1%, with relative standard deviations (RSDs) from 0.33% to 10.81%. This method was suitable for the detection of BAs in aquatic products and their products. |
| Databáze: | OpenAIRE |
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