Comparison of the uptake mechanisms of zwitterionic and negatively charged liposomes by HeLa cells
| Autor: | Catharina Reker-Smit, Anna Salvati, Keni Yang, Daphne Montizaan |
|---|---|
| Přispěvatelé: | Nanomedicine & Drug Targeting, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Biopharmaceuticals, Discovery, Design and Delivery (BDDD) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biomedical Engineering
Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Endocytosis HeLa 03 medical and health sciences Humans General Materials Science Actin 030304 developmental biology 0303 health sciences Liposome biology Chemistry Pinocytosis Flow Cytometry 021001 nanoscience & nanotechnology biology.organism_classification Kinetics Liposomes PEGylation Biophysics Molecular Medicine RNA Interference Nanocarriers 0210 nano-technology HeLa Cells Protein adsorption |
| Zdroj: | Nanomedicine-Nanotechnology biology and medicine, 30:102300. ELSEVIER SCIENCE BV Nanomedicine: Nanotechnology, Biology and Medicine |
| ISSN: | 1549-9634 |
| Popis: | Zwitterionic molecules are used as an alternative to PEGylation to reduce protein adsorption on nanocarriers. Nonetheless, little is known on the effect of zwitterionic modifications on the mechanisms cells use for nanocarrier uptake. In this study, the uptake mechanism of liposomes containing zwitterionic or negatively charged lipids was characterized using pharmacological inhibitors and RNA interference on HeLa cells to block endocytosis. As expected, introducing zwitterionic lipids reduced protein adsorption in serum, as well as uptake efficiency. Blocking clathrin-mediated endocytosis strongly decreased the uptake of the negatively charged liposomes, but not the zwitterionic ones. Additionally, inhibition of macropinocytosis reduced uptake of both liposomes, but blocking actin polymerization had effects only on the negatively charged ones. Overall, the results clearly indicated that the two liposomes were internalized by HeLa cells using different pathways. Thus, introducing zwitterionic lipids affects not only protein adsorption and uptake efficiency, but also the mechanisms of liposome uptake by cells. |
| Databáze: | OpenAIRE |
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