Identification and Quantification of Stilbenes in Fruits of Transgenic Tomato Plants (Lycopersicon esculentum Mill.) by Reversed Phase HPLC with Photodiode Array and Mass Spectrometry Detection
| Autor: | Antonella De Rossi, Isabella Nicoletti, Giovanna Giovinazzo, Danilo Corradini |
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| Rok vydání: | 2007 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Electrospray ionization stilbenes Mass spectrometry High-performance liquid chromatography Lycopersicon Solanum lycopersicum Phenols Lycopersicon esculentum Mill Genetically modified tomato Chromatography High Pressure Liquid Flavonoids Chromatography biology Chemistry fungi metabolic profiling Polyphenols food and beverages General Chemistry Reversed-phase chromatography Plants Genetically Modified biology.organism_classification HPLC/PDA/MS Transgenic tomato plants Polyphenol Fruit General Agricultural and Biological Sciences |
| Zdroj: | Journal of agricultural and food chemistry 55 (2007): 3304–3311. doi:10.1021/jf063175m info:cnr-pdr/source/autori:Nicoletti Isabella; De Rossi Antonella; Giovinazzo Giovanna; Corradini Danilo/titolo:Identification and Quantification of Stilbenes in Fruits of Transgenic Tomato Plants (Lycopersicon esculentum Mill.) by Reversed Phase HPLC with Photodiode Array and Mass Spectrometry Detection/doi:10.1021%2Fjf063175m/rivista:Journal of agricultural and food chemistry/anno:2007/pagina_da:3304/pagina_a:3311/intervallo_pagine:3304–3311/volume:55 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf063175m |
| Popis: | Reversed-phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) and mass spectrometry (MS) detection was employed to study the accumulation of stilbenes and other naturally occurring polyphenol intermediates of flavonoid pathway in tomato fruits of plants genetically modified to synthesize resveratrol. The transgenic tomato fruits were obtained by overexpression of a grapevine gene encoding the enzyme stilbene synthase in tomato plants (Lycopersicon esculentum Mill.). Stilbenes and flavonoids, either glycosylated or free, were simultaneosly identified by electrospray interface (ESI)-MS in negative ionization mode and were quantified by PDA detection at the wavelength corresponding to their maximum absorbance. The two detectors were coupled online with an HPLC system utilizing a narrow-bore C18 reversed-phase column, which was eluted by a multistep gradient of increasing concentration of acetonitrile in water containing 0.5% (v/v) formic acid. The results of these analysis revealed that the genetic modification of the tomato plants originated different levels of accumulation of four stilbenes (i.e., trans- and cis-piceid and trans- and cis-resveratrol) in their fruit depending on the stages of ripening. Either at immature or at mature stages of ripening the stilbenes were preferentially accumulated in the fruit peel as the glycosylated form. The highest amount of trans-piceid and trans-resveratrol were found in the peel of fruits harvested at mature stage of ripening. The variations in the levels of rutin, naringenin, and chlorogenic acid found in the samples extracted from the fruits of transgenic tomato plants, in comparison to that determined in the control lines, seemed to be related to the genetic transformation, whose effect on the flavonoid biosynthetic pathway needs to be elucidated by additional studies. |
| Databáze: | OpenAIRE |
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