Embryonic Expression and Function of the Chemokine SDF-1 and Its Receptor, CXCR4
| Autor: | James Palis, Kathleen M. Maltby, Judeth K. McGann, Kathleen E. McGrath, Anne D. Koniski |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
CCR1
Receptors CXCR4 CCR2 cerebellum HIV coreceptor C-C chemokine receptor type 6 Biology neuronal Embryonic and Fetal Development Mice 03 medical and health sciences Chemokine receptor 0302 clinical medicine Cell Movement Animals CXC chemokine receptors gastrulation Molecular Biology CXCL16 030304 developmental biology Mice Inbred ICR 0303 health sciences Gene Expression Regulation Developmental conotruncal Cell Differentiation Cell Biology Embryo Mammalian Molecular biology Chemokine CXCL12 Hematopoiesis Cell biology XCL2 in situ hybridization CC chemokine receptors Chemokines CXC 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. (2):442-456 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1999.9405 |
| Popis: | Directed cell movement is integral to both embryogenesis and hematopoiesis. In the adult, the chemokine family of secreted proteins signals migration of hematopoietic cells through G-coupled chemokine receptors. We detected embryonic expression of chemokine receptor messages by RT-PCR with degenerate primers at embryonic day 7.5 (E7.5) or by RNase protection analyses of E8.5 and E12.5 tissues. In all samples, the message encoding CXCR4 was the predominate chemokine receptor detected, particularly at earlier times (E7.5 and E8.5). Other chemokine receptor messages (CCR1, CCR4, CCR5, CCR2, and CXCR2) were found in E12.5 tissues concordant temporally and spatially with definitive (adult-like) hematopoiesis. Expression of CXCR4 was compared with that of its only known ligand, stromal cell-derived factor-1 (SDF-1), by in situ hybridization. During organogenesis, these genes have dynamic and complementary expression patterns particularly in the developing neuronal, cardiac, vascular, hematopoietic, and craniofacial systems. Defects in the first four of these systems have been reported in CXCR4- and SDF-1-deficient mice. Our studies suggest new potential mechanisms for some of these defects as well as additional roles beyond the scope of the reported abnormalities. Earlier in development, expression of these genes correlates with migration during gastrulation. Migrating cells (mesoderm and definitive endoderm) contain CXCR4 message while embryonic ectoderm cells express SDF-1. Functional SDF-1 signaling in midgastrula cells as well as E12.5 hematopoietic progenitors was demonstrated by migration assays. Migration occurred with an optimum dose similar to that found for adult hematopoietic cells and was dependent on the presence of SDF-1 in a gradient. This work suggests roles for chemokine signaling in multiple embryogenic events. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|