Distribution and characterization of immunoreactive porcine C-type natriuretic peptide
| Autor: | Shuzo Ueda, Shigeru Matsukura, Kenji Kangawa, Naoto Minamino, Masahito Aburaya, Hisayuki Matsuo |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Cerebellum Swine medicine.drug_class Central nervous system Radioimmunoassay Biophysics Nerve Tissue Proteins Peptide hormone Biochemistry Immunoenzyme Techniques Internal medicine medicine Natriuretic peptide Animals Molecular Biology Chromatography High Pressure Liquid Brain Chemistry Antiserum Chemistry Immune Sera Natriuretic Peptide C-Type Cell Biology NPR2 medicine.anatomical_structure Endocrinology Spinal Cord Organ Specificity Chromatography Gel Quantitative analysis (chemistry) Atrial Natriuretic Factor |
| Zdroj: | Biochemical and Biophysical Research Communications. 175:759-767 |
| ISSN: | 0006-291X |
| Popis: | C-type natriuretic peptide (CNP) is a new member of the natriuretic peptide family recently identified in porcine brain (1). We raised an antiserum against porcine CNP and set up a radioimmunoassay (RIA) for CNP. Using this RIA system, distribution of immunoreactive (ir-) CNP in porcine tissue was measured and compared with that of ir-atrial natriuretic peptide (ANP) and ir-brain natriuretic peptide (BNP). Tissue concentration of ir-CNP in brain was the highest of the three natriuretic peptides at about 0.79 pmol/g wet wt. CNP was present in medulla-pons in high concentration, with a significant concentration detected in cerebellum. In contrast, ir-CNP was not detected in peripheral tissue, including heart, in a significant concentration. These data demonstrated sharp contrasts in the distribution of the three natriuretic peptides, suggesting that CNP is a natriuretic peptide functioning in the central nervous system. |
| Databáze: | OpenAIRE |
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