7-Hydroxylation of dehydroepiandrosterone by a human lymphoblastoid cell line
| Autor: | N. Kraft, K.N. Wynne |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
medicine.medical_specialty
Dehydroepiandrosterone Biology Hydroxylation Lymphocyte Activation Biochemistry Molecular biology In vitro Cell Line chemistry.chemical_compound Endocrinology chemistry Internal medicine Mitogen-activated protein kinase Steroid Hydroxylases medicine biology.protein Liberation Tritium Specific activity Lymphocytes Incubation |
| Zdroj: | Journal of steroid biochemistry. 9(12) |
| ISSN: | 0022-4731 |
| Popis: | The enzymic liberation of tritium from [7-3H]-dehydroepiandrosterone was used as an in vitro assay for the possible presence of 7-hydroxylase in human lymphocytes. Indications of low activity were found in mitogen transformed lymphocytes from healthy donors and in lymphocytes from some patients with acute and chronic lymphocytic leukaemia. However, a lymphoblastoid cell line (McB) derived from a subject with Hodgkin's Disease proved exceptionally active when assessed by this procedure. An incubation of 108 McB cells with [7-3H]-dehydroepiandrosterone produced [3H]-7α-hydroxyde-hydroepiandrosterone in 12.2% yield. Identification of this product was established by crystallizations to constant specific activity. |
| Databáze: | OpenAIRE |
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