Evaluation of the intranasal challenge route in mice as a mucosal model for Candida albicans infection
| Autor: | Frances Bowe, George Booth, Gordon Dougan, William L. McPheat, Patricia Londoño, Xiao M. Gao |
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| Rok vydání: | 1998 |
| Předmět: |
Cellular immunity
Spleen Microbiology Mice Immune system Immunity Candida albicans medicine Splenocyte Animals Administration Intranasal Antibodies Fungal Immunity Cellular Mice Inbred BALB C biology Lung Diseases Fungal Candidiasis biology.organism_classification Corpus albicans Disease Models Animal Kinetics Nasal Mucosa medicine.anatomical_structure Mice Inbred DBA Immunology Antibody Formation Nasal administration Disease Susceptibility |
| Zdroj: | Microbiology (Reading, England). 144 |
| ISSN: | 1350-0872 |
| Popis: | The intranasal route was used to study Candida albicans infections in mice. Mice from two different inbred strains were challenged intranasally with C. albicans and the level of local and systemic colonization was monitored. DBA/2 mice were highly susceptible to challenge and viable C. albicans disseminated from the lungs to deeper tissues, including kidneys, liver and spleen within 48 h. In contrast, in BALB/c mice challenged in the same manner, C. albicans were retained within the lungs and cleared. Local and systemic anti-C. albicans immune responses were investigated. BALB/c mice exhibited higher titres of serum and mucosal anti-C. albicans IgA than DBA/2 mice. Splenocytes from BALB/c mice, but not from DBA/2 mice, produced detectable levels of interleukin-4 and -5 following stimulation with C. albicans antigens. Both DBA/2- and BALB/c-derived splenocytes produced interferon-γ and interleukin-10 in response to similar stimulation. In conclusion, the intranasal route provided a simple, non-invasive murine model for investigating C. albicans infection through mucosal surfaces. |
| Databáze: | OpenAIRE |
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