17beta-estradiol and progesterone prevent cuprizone provoked demyelination of corpus callosum in male mice
| Autor: | Sámuel Komoly, Cordian Beyer, Alena Braun, Sonja Johann, Péter Ács, Akvile Norkute, Tim Clarner, Markus Kipp, Zoltán Berente |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Receptor Platelet-Derived Growth Factor alpha medicine.drug_class Biology Corpus callosum Neuroprotection Drug Administration Schedule Corpus Callosum Cellular and Molecular Neuroscience Cuprizone Mice Internal medicine Glial Fibrillary Acidic Protein medicine Animals Remyelination Insulin-Like Growth Factor I Cytoskeleton Progesterone Analysis of Variance Estradiol Multiple sclerosis Estrogens medicine.disease Magnetic Resonance Imaging Oligodendrocyte Astrogliosis Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Endocrinology Neurology Gene Expression Regulation Estrogen Myelin Proteins Hormone Demyelinating Diseases |
| Zdroj: | Glia. 57(8) |
| ISSN: | 1098-1136 |
| Popis: | Sex hormones, for example, estrogen and progesterone, are thought to affect and delay progression of multiple sclerosis (MS) in pregnant women. Although both steroid hormones are neuroprotective in the brain and elevated during pregnancy, only estrogen was tested in clinical trials. To evaluate the role of 17β-estradiol (E) and progesterone (P) in prevention demyelination, young adult male mice were fed with cuprizone for a defined time interval and simultaneously treated with steroids by repeated injections into the neck region. The status of myelination was analyzed by magnetic resonance imaging and conventional histological staining. The individual application of E and P resulted only in a moderate prevention of demyelination in the corpus callosum (CC). The combined treatment with both steroid hormones counteracted the process of demyelination. Expression of the mature (PLP and MBP) and premature (PDGF-α-R) oligodendrocyte markers were significantly increased after hormone application in the affected CC. In addition, both hormones stimulated astrogliosis and the expression of IGF-1. Microglial invasion in demyelinated CC was pronounced and additionally localized in the midline of CC after hormone treatment. These data show that sex steroids can protect the brain from demyelination and stimulate remyelination. It appears that only the administration of both hormones is fully effective. The beneficial steroid effect requires interactions with oligodendrocytes possibly by preventing their degeneration or recruitment from precursor cells which are stimulated to remyelinated fibers. The positive hormonal influence on myelination in the CNS may be a future therapeutically strategy for the treatment of MS. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text