Insulin is necessary for the hypertrophic effect of cholecystokinin-octapeptide following acute necrotizing experimental pancreatitis
| Autor: | Péter Hegyi, József Németh, Zoltán Rakonczay, Réka Sári, Norbert Farkas, Tamás Takács, Csaba Góg, László Czakó, János Lonovics |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Trypsinogen medicine.medical_treatment digestive system Sincalide Diabetes Mellitus Experimental chemistry.chemical_compound Internal medicine Diabetes mellitus medicine Animals Hypoglycemic Agents Insulin Regeneration Amylase Rats Wistar biology Pancreatitis Acute Necrotizing business.industry digestive oral and skin physiology Gastroenterology General Medicine Streptozotocin medicine.disease Rats Endocrinology chemistry biology.protein Pancreatitis Acute pancreatitis Brief Reports business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v10.i15.2275 |
| Popis: | AIM: In previous experiments we have demonstrated that by administering low doses of cholecystokinin-octapeptide (CCK-8), the process of regeneration following L-arginine (Arg)-induced pancreatitis is accelerated. In rats that were also diabetic (induced by streptozotocin, STZ), pancreatic regeneration was not observed. The aim of this study was to deduce whether the administration of exogenous insulin could in fact restore the hypertrophic effect of CCK-8 in diabetic-pancreatitic rats. METHODS: Male Wistar rats were used for the experiments. Diabetes mellitus was induced by administering 60 mg/kg body mass of STZ intraperitoneally (i.p.), then, on d 8, pancreatitis was induced by 200 mg/100 g body mass Arg i.p. twice at an interval of 1 h. The animals were injected subcutaneously twice daily (at 7 a.m. and 7 p.m.) with 1 μg/kg of CCK-8 and/or 2 IU mixed insulin (300 g/L short-action and 700 g/L intermediate-action insulin) for 14 d after pancreatitis induction. Following this the animals were killed and the serum amylase, glucose and insulin levels as well as the plasma glucagon levels, the pancreatic mass/body mass ratio (pm/bm), the pancreatic contents of DNA, protein, amylase, lipase and trypsinogen were measured. Pancreatic tissue samples were examined by light microscopy on paraffin-embedded sections. RESULTS: In the diabetic-pancreatitic rats treatment with insulin and CCK-8 significantly elevated pw/bm and the pancreatic contents of protein, amylase and lipase vs the rats receiving only CCK-8 treatment. CCK- 8 administered in combination with insulin also elevated the number of acinar cells with mitotic activities, whereas CCK- 8 alone had no effect on laboratory parameters or the mitotic activities in diabetic-pancreatitic rats. CONCLUSION: Despite the hypertrophic effect of CCK-8 being absent following acute pancreatitis in diabetic-rats, the simultaneous administration of exogenous insulin restored this effect. Our results clearly demonstrate that insulin is necessary for the hypertrophic effect of low-doses of CCK-8 following acute pancreatitis. |
| Databáze: | OpenAIRE |
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