The Pacific White Shrimp β-actin Promoter: Functional Properties and the Potential Application for Transduction System Using Recombinant Baculovirus
| Autor: | Yuanan Lu, Jianhai Xiang, Si Sun, Guangzhou Zhou, Hsin-I Tong, Yingli Shi, Tetsuzan Benny Ron, Wen Kang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Baculoviridae Cytomegalovirus Simian virus 40 Spodoptera Bioinformatics Applied Microbiology and Biotechnology Cell Line 03 medical and health sciences White spot syndrome virus 1 Penaeidae Genes Reporter Salmon Transduction Genetic Polyhedrin Animals Humans Luciferase Luciferases Promoter Regions Genetic Gene Zebrafish Regulation of gene expression Base Sequence 030102 biochemistry & molecular biology biology Promoter biology.organism_classification Molecular biology Actins Turtles 030104 developmental biology Gene Expression Regulation Cell culture Ectopic expression Genetic Engineering HeLa Cells Tilapia |
| Zdroj: | Marine Biotechnology. 18:349-358 |
| ISSN: | 1436-2236 1436-2228 |
| DOI: | 10.1007/s10126-016-9700-1 |
| Popis: | A newly isolated Pacific white shrimp (Litopenaeus vannamei) beta-actin promoter SbaP and its derivative compact construct SbaP (ENX) have recently been demonstrated to promote ectopic gene expression in vitro and in vivo. To further explore the potential transduction application, this newly isolated shrimp promoter SbaP was comparatively tested with cytomegalovirus (CMV), simian virus 40 (SV40), polyhedrin (Polh), and white spot syndrome virus immediate early gene 1 (WSSV ie1) four constitutive promoters and a beta-actin promoter (TbaP) from tilapia fish to characterize its promoting function in eight different cell lines. Luciferase quantitation assays revealed that SbaP can drive luciferase gene expression in all eight cell lines including sf21 (insect), PAC2 (zebrafish), EPC (carp), CHSE-214 (chinook salmon), GSTEF (green sea turtle), MS-1 (monk seal), 293T (human), and HeLa (human), but at different levels. Comparative analysis revealed that the promoting activity of SbaP was lower ( |
| Databáze: | OpenAIRE |
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