Temperature-dependent basal tone in isolated human saphenous veins: implication of TP-receptors

Autor: Serge Simonet, Tony Verbeuren, Edith Bonhomme, Jean-Noël Fabiani
Rok vydání: 2000
Předmět:
medicine.medical_specialty
Contraction (grammar)
Endothelium
Receptors
Thromboxane
Carbazoles
In Vitro Techniques
Dinoprost
Nitric Oxide
Synaptic Transmission
Thromboxane A2
chemistry.chemical_compound
Norepinephrine
Internal medicine
medicine
Humans
Vasoconstrictor Agents
Pharmacology (medical)
Drug Interactions
Saphenous Vein
Receptor
Phentolamine
Adrenergic alpha-Antagonists
Pharmacology
F2-Isoprostanes
Sulfonamides
biology
Endothelins
Temperature
Anatomy
Epoprostenol
medicine.anatomical_structure
Endocrinology
chemistry
Vasoconstriction
15-Hydroxy-11 alpha
9 alpha-(epoxymethano)prosta-5
13-dienoic Acid
Circulatory system
cardiovascular system
biology.protein
Verapamil
Calcium
Vascular Resistance
Cyclooxygenase
Endothelium
Vascular
Platelet Aggregation Inhibitors
medicine.drug
Blood vessel
Zdroj: Fundamentalclinical pharmacology. 14(5)
ISSN: 0767-3981
Popis: Cutaneous blood vessels are very sensitive to changes in environmental temperature. The influence of variations in local temperature on the mechanisms involved in the basal tone, present in isolated human saphenous veins has not yet been studied. In the present study, segments with and without endothelium of human saphenous veins obtained from coronary bypass surgery patients were mounted for isometric tension recording in oxygenated physiological salt solution (PSS). After stabilisation of the basal tone, the local temperature was rapidly either decreased from 37 degrees C to 24 degrees C (cooling) or increased from 37 degrees C to 42 degrees C (warming). When antagonists or inhibitors were used the preparations were incubated for 30 min with the drugs. During basal conditions, cooling caused relaxations of the saphenous vein segments with endothelium and warming caused contractions; the absence of the endothelium did not modify these responses. In veins without endothelium, the warming-induced contractions were significantly inhibited by verapamil (10 microM) and by the antagonist of TP-receptors (receptors for thromboxane A2) Bay u 3405 (1 microM). The warming induced contractions were not affected by cyclooxygenase or lipoxygenase inhibition. At 37 degrees C, the isoprostanes (8-iso-PGE2 and 8-iso-PGF2alpha) induced potent contractions that were significantly inhibited by Bay u 3405 (1 microM). The data show that a basal tone is present in isolated resting human saphenous vein segments at 37 degrees C. This basal tone is decreased by local cooling and enhanced by local warming and is not dependent on the presence of the endothelium. The warming-induced contraction of the veins is mediated by a non-cyclooxygenase, non-lipoxygenase metabolite (isoprostane?) that interacts with TP-receptors and via an extracellular calcium-dependent pathway.
Databáze: OpenAIRE
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