Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers
| Autor: | R Kandar, X Stramova |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Economics and Econometrics Semen Semen analysis medicine.disease_cause High-performance liquid chromatography Sensitivity and Specificity Lipid peroxidation chemistry.chemical_compound Malondialdehyde Materials Chemistry Media Technology medicine Humans Sperm motility Chromatography High Pressure Liquid Infertility Male Chromatography medicine.diagnostic_test Smoking Forestry Sperm Semen Analysis Cross-Sectional Studies chemistry Sperm Motility Lipid Peroxidation Reactive Oxygen Species Oxidative stress Biomarkers |
| Zdroj: | Bratislavske lekarske listy. 116(1) |
| ISSN: | 0006-9248 |
| Popis: | OBJECTIVES The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups. BACKGROUND Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level. Malondialdehyde has been thought to have cytotoxic and damaging effects. METHODS Semen samples were obtained from male partners of couples requesting a fertility evaluation. Malondialdehyde was derivatized with 2-thiobarbituric acid. The malondialdehyde-2-thiobarbituric acid complex was determined by liquid chromatography with fluorescence detection. The mobile phase consisted of 20% ethanol in 25-mmol/L potassium dihydrogenphosphate (v/v), pH 6.00 ± 0.05. RESULTS Analytical performance was satisfactory. Malondialdehyde levels were as follows: 1.50 ± 0.55 µmol/L in all patients, 1.40 ± 0.57 µmol/L in smokers, and 1.50 ± 0.53 µmol/L in non-smokers. CONCLUSION The method presented here is sensitive and accurate for seminal plasma malondialdehyde determination. Our results showed a relationship between sperm motility and the malondialdehyde level in all patients and non-smokers. Malondialdehyde may induce poor sperm functionality and negatively affect the fertilization processes (Tab. 1, Fig. 1, Ref. 23). |
| Databáze: | OpenAIRE |
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