Sodium alendronate loaded poly(l -lactide- co-glycolide) microparticles immobilized on ceramic scaffolds for local treatment of bone defects
| Autor: | Håvard J. Haugen, Sina Rößler, Elżbieta Pamuła, Katarzyna Reczyńska, Cornelia Wolf-Brandstetter, Łucja Rumian, Dieter Scharnweber, Hanna Tiainen |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology Chemistry Acid phosphatase Osteoblast 02 engineering and technology 021001 nanoscience & nanotechnology Bone tissue Bone resorption Bone remodeling Cell-Derived Microparticles Biomaterials 03 medical and health sciences medicine.anatomical_structure Osteoclast medicine biology.protein 0210 nano-technology 030304 developmental biology Biomedical engineering Tartrate-resistant acid phosphatase |
| Zdroj: | Regenerative Biomaterials. 8 |
| ISSN: | 2056-3426 2056-3418 |
| DOI: | 10.1093/rb/rbaa012 |
| Popis: | Bone tissue regeneration in critical-size defects is possible after implantation of a 3D scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and healing. Sodium alendronate (Aln), a widely used anti-osteoporosis drug, exhibits strong inhibitory effect on bone resorption performed by osteoclasts. Thus, we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide scaffolds and poly(l-lactide-co-glycolide) microparticles (MPs) loaded with Aln. The MPs were effectively attached to the surface of the scaffolds’ pore walls by human recombinant collagen. Drug release from the scaffolds was characterized by initial burst (24 ± 6% of the drug released within first 24 h) followed by a sustained release phase (on average 5 µg of Aln released per day from Day 3 to Day 18). In vitro tests evidenced that Aln at concentrations of 5 and 2.5 µg/ml was not cytotoxic for MG-63 osteoblast-like cells (viability between 81 ± 6% and 98 ± 3% of control), but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells, as shown by reduced fusion capability and decreased tartrate-resistant acid phosphatase 5b activity (56 ± 5% reduction in comparison to control after 8 days of culture). Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis, reducing osteoclast activity, but not affecting osteoblast functions, which may be beneficial in the treatment of critical-size bone tissue defects. |
| Databáze: | OpenAIRE |
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