[Effect of pingxian granules on protein expression of apoptosis regulatory genes in hippocampus of pentylenetetrazol-induced epileptic model rats]
| Autor: | Xiaoli Cheng, Yu Wang, Yali She, Baolong Mu, Rong Tian, Yuxin Jia |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Pathology Apoptosis Biology Hippocampus Epilepsy Internal medicine medicine Hippocampus (mythology) Animals Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Pentylenetetrazol Rats Wistar Regulator gene medicine.disease Immunohistochemistry Rats Endocrinology Complementary and alternative medicine Pentylenetetrazole Phenobarbital Female Perfusion medicine.drug Drugs Chinese Herbal |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 37(9) |
| ISSN: | 1001-5302 |
| Popis: | OBJECTIVE To observe the effect of Pingxian granules on the protein expression of apoptosis regulatory genes Bcl-2 and Bax in the hippocampus of epileptic model rats and study the molecular biological mechanism of the anti-epileptic effect of Pingxian granules. METHOD Totally 60 45-days-old Wistar rats were selected and then randomly assigned into 5 groups: the normal control group, the model group, the positive control group, the Pingxian high dose group and the Pingxian low dose group, with 12 in each group. Except the normal control group, all the groups were intraperitoneally injected with 35 mg x kg(-1) pentylenetetrazol to establish the models of epilepsy. The Pingxian high dose (1.66 g x mL(-1)) and low dose (0.42 g x mL(-1)) groups were intragastrically infused with Pingxian granules 2 mL x d(-1). The positive control group received 3.6 g x L(-1) phenobarbital suspension by gastric perfusion. The normal group and the model group were drenched with distilled water, 2 mL x d(-1), for 5 weeks. Bcl-2 and Bax protein positive cells were labeled with immunohistochemical SABC at 3, 5 w. RESULT (1) Rats in the model group appeared the epileptic behavior at the 1st week, and became serious with the kindle frequency; grade VIepileptic behavior appeared at the 4th week. The attack frequency and grade of the Pingxian group were less and lower, the highest grade were only IV, and there were no significant differences in the attack grade and frequency. (2) With the increase in kindle frequency, the model group showed a notable decrease in the Bcl-2 expression compared with the normal control group at the 3rd and 5th weekend (P < 0.01), but a significant increase in Bax protein expression (P |
| Databáze: | OpenAIRE |
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