Rescue of Vasopressin Synthesis in Magnocellular Neurons of the Supraoptic Nucleus Normalises Acute Stress-Induced Adrenocorticotropin Secretion and Unmasks an Effect on Social Behaviour in Male Vasopressin-Deficient Brattleboro Rats
| Autor: | Bibiána Török, Péter Csikota, Anna Fodor, Diána Balázsfi, Szilamér Ferenczi, Kornél Demeter, Zsuzsanna E. Tóth, Katalin Könczöl, Judith Camats Perna, Imre Farkas, Krisztina J. Kovács, József Haller, Mario Engelmann, Dóra Zelena |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
Hypothalamo-Hypophyseal System endocrine system vasopressin QH301-705.5 Corticotropin-Releasing Hormone Vasopressins adrenocorticotropin Catalysis social behaviour Inorganic Chemistry Adrenocorticotropic Hormone Animals RNA Messenger Biology (General) Physical and Theoretical Chemistry medial amygdala Social Behavior QD1-999 Molecular Biology Spectroscopy corticosterone c-Fos supraoptic nucleus Neurons urogenital system Organic Chemistry Brain Rats Brattleboro General Medicine Computer Science Applications Rats Chemistry nervous system Basal Nucleus of Meynert Corticosterone Supraoptic Nucleus hormones hormone substitutes and hormone antagonists Paraventricular Hypothalamic Nucleus |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1357 International Journal of Molecular Sciences, Vol 23, Iss 1357, p 1357 (2022) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23031357 |
| Popis: | The relevance of vasopressin (AVP) of magnocellular origin to the regulation of the endocrine stress axis and related behaviour is still under discussion. We aimed to obtain deeper insight into this process. To rescue magnocellular AVP synthesis, a vasopressin-containing adeno-associated virus vector (AVP-AAV) was injected into the supraoptic nucleus (SON) of AVP-deficient Brattleboro rats (di/di). We compared +/+, di/di, and AVP-AAV treated di/di male rats. The AVP-AAV treatment rescued the AVP synthesis in the SON both morphologically and functionally. It also rescued the peak of adrenocorticotropin release triggered by immune and metabolic challenges without affecting corticosterone levels. The elevated corticotropin-releasing hormone receptor 1 mRNA levels in the anterior pituitary of di/di-rats were diminished by the AVP-AAV-treatment. The altered c-Fos synthesis in di/di-rats in response to a metabolic stressor was normalised by AVP-AAV in both the SON and medial amygdala (MeA), but not in the central and basolateral amygdala or lateral hypothalamus. In vitro electrophysiological recordings showed an AVP-induced inhibition of MeA neurons that was prevented by picrotoxin administration, supporting the possible regulatory role of AVP originating in the SON. A memory deficit in the novel object recognition test seen in di/di animals remained unaffected by AVP-AAV treatment. Interestingly, although di/di rats show intact social investigation and aggression, the SON AVP-AAV treatment resulted in an alteration of these social behaviours. AVP released from the magnocellular SON neurons may stimulate adrenocorticotropin secretion in response to defined stressors and might participate in the fine-tuning of social behaviour with a possible contribution from the MeA. |
| Databáze: | OpenAIRE |
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