Uniformly-sized, molecularly imprinted polymers for (-)-epigallocatechin gallate, -epicatechin gallate and -gallocatechin gallate by multi-step swelling and polymerization method
| Autor: | Hiromi Tabo, Haruyo Sambe, Akio Sugimoto, Masaki Ichitani, Takanobu Takihara, Jun Haginaka |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chromatography
Tea Organic Chemistry Molecularly imprinted polymer food and beverages General Medicine Gallate Green tea extract Epigallocatechin gallate complex mixtures Biochemistry Catechin Analytical Chemistry chemistry.chemical_compound Epicatechin gallate chemistry Polymerization Gallocatechin gallate Molecular imprinting Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. A. 1156(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Uniformly-sized, molecularly imprinted polymers (MIPs) for (-)-epigallocatechin gallate (EGCg), -epicatechin gallate (ECg) and -gallocatechin gallate (GCg) were prepared by a multi-step swelling and polymerization method using 2-vinylpyridine as a functional monomer, ethylene glycol dimethacrylate as a cross-linker and cyclohexanol as a porogen. Molecular recognition abilities of the obtained MIPs were evaluated in liquid chromatography using a mixture of ethanol and water, or ethanol as the eluent. Each MIP gave the highest molecular recognition ability for the respective template molecule. In addition, (-)-EGCg and -ECg had the same configuration (2R,3R) at positions 2 and 3, and therefore resulting in high cross reactivity each other. However, (-)-GCg, which has different configuration at position 2 with (-)-EGCg and -ECg, showed low cross reactivity with them. On the other hand, those MIPs showed no molecular recognition against (-)-epigallocatechin and -epicatechin, which have no gallate group at position 3. These results indicate that the MIPs prepared can recognize configuration at position 2 and a gallate group at position 3. Furthermore, the MIP for (-)-GCg could be successfully used for isolating (-)-EGCg and -ECg from green tea extract. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect