Evaluation of the chemical consistency of Yin‐Chen‐Hao‐Tang prepared by combined and separated decoction methods using high‐performance liquid chromatography and quadrupole time‐of‐flight mass spectrometry coupled with multivariate statistical analysis
| Autor: | Zhaolin Sun, Huan Liu, Qiang Tian, Fang Liu, Zhou Xu, Lin Ma, Chenggang Huang, Mingcang Chen, Hao Yin, Zhixiong Li |
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| Rok vydání: | 2019 |
| Předmět: |
Filtration and Separation
Decoction Mass spectrometry 01 natural sciences High-performance liquid chromatography Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Caffeic Acids Chlorogenic acid Scopoletin Caffeic acid Iridoids Chromatography High Pressure Liquid 030304 developmental biology 0303 health sciences Chromatography 010401 analytical chemistry Discriminant Analysis 0104 chemical sciences chemistry Multivariate Analysis Principal component analysis Time-of-flight mass spectrometry Drugs Chinese Herbal |
| Zdroj: | Journal of Separation Science. 42:1664-1675 |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.201800961 |
| Popis: | In this study, Yin-Chen-Hao-Tang prepared by two decoction methods, namely, combined decoction (modern decoction method) and separated decoction (traditional decoction method), was analyzed by high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. The acquired datasets containing sample codes, tR -m/z pairs and ion intensities were processed with multivariate statistical analyses, such as principal component analysis and an orthogonal partial least squared discriminant analysis model, to globally compare the chemical differences between the different decoction samples. Then, the chemical differences between the combined and separated decoctions were screened out by S-plots generated from the orthogonal partial least squared discriminant analysis model and compared with chemical information from an established in-house library. The six components that contributed the most to the chemical differences were identified as chlorogenic acid, caffeic acid, geniposide, genipin, scopoletin, and 3,5-dicaffeoylquinic acid. The concentrations of genipin and caffeic acid from the separated decoction were higher than those from the combined decoction, indicating that the separated decoction may present a stronger hepatoprotective effect. However, the results still require further investigation through pharmacological and clinical studies. Our findings not only establish a strategy to evaluate chemical consistency of Yin-Chen-Hao-Tang but also provide the scientific basis for using traditional separated decoction method. |
| Databáze: | OpenAIRE |
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