Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages

Autor: Kevin S. W. Tan, Kate Breyer, Georges Snounou, Clemens H. M. Kocken, Anne Elliot, Bruce Russell, Varakorn Kosaisavee, Bryan K. S. Yeung, Bee Huat Tan, Adeline C. Y. Chua, Rossarin Suwanarusk, Siti Nurdiana Abas, Rou Zhang, Anne-Marie Zeeman, Jee Sun Cho, Benoit Malleret, Laurent Rénia, Roger Le Grand, Dennis E. Kyle, Mary R. Galinski, Christophe Bodenreider, Nathalie Dereuddre-Bosquet, Chester J. Joyner, François Nosten, Dominique Mazier, Szczepan W Baran, Pablo Bifani, Amber Lange, Jessica Jie Ying Ong, Thierry T. Diagana, Steven P. Maher, Caitlin A. Cooper, Andy M. Yip
Přispěvatelé: Institut des Maladies Emergentes et des Thérapies Innovantes (IMETI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Global Health Division, Menzies School of Health Research, Mahidol Oxford Tropical Medicine Research Unit, University of Oxford [Oxford]-Mahidol University [Bangkok], Centre d'Immunologie et de Maladies Infectieuses (CIMI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Immunologie des Maladies Virales et Autoimmunes (IMVA - U1184), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biomedical Primate Research Centre [Rijswijk] (BPRC), Institut Cochin (UMR_S567 / UMR 8104), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Novartis Institute for Tropical Diseases (NITD), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Oxford-Mahidol University [Bangkok], ANR-17-CE13-0025,IMHyp,Recherches sur l'Hypnozoïte du Paludisme(2017)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications
Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-11332-4⟩
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-11332-4⟩
ISSN: 2041-1723
Popis: The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts.
Present understanding of Plasmodium vivax biology is hampered by its inability to grow in vitro. Here, the authors developed an in vitro culture of its simian counterpart, P. cynomolgi, which shares morphological and phenotypic similarities with P. vivax, initiating a new phase in vivax research.
Databáze: OpenAIRE
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