Detection of interferon regulatory factor-1 in lamina propria mononuclear cells in Crohn's disease
| Autor: | Elizabeth E. Mannick, Zhiyun Liu, R. Brown, E. Schmidt-Sommerfeld, A Delgado, M Clavell, H. Correa-Gracian, J. N. Udall, Randall D. Craver |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Adolescent Colon Inflammatory bowel disease Peripheral blood mononuclear cell Pathogenesis Immunoenzyme Techniques Crohn Disease medicine Humans Biotinylation Colitis Child Lamina propria Crohn's disease Tissue Embedding business.industry Gastroenterology medicine.disease Phosphoproteins Ulcerative colitis DNA-Binding Proteins medicine.anatomical_structure Paraffin Child Preschool Pediatrics Perinatology and Child Health Immunology Colitis Ulcerative business Interferon regulatory factors Interferon Regulatory Factor-1 Transcription Factors |
| Zdroj: | Journal of pediatric gastroenterology and nutrition. 30(1) |
| ISSN: | 0277-2116 |
| Popis: | Background The transcription factor, interferon regulatory factor (IRF)-1, is stimulated by interferon-gamma and regulates the expression of several genes implicated in the pathogenesis of inflammatory bowel disease, including interleukin-6, major histocompatibility complex class II molecules, and inducible nitric oxide synthase. Interferon regulatory factor-1 also stimulates naive CD4+ T-cells to differentiate into T-helper-1 cells, the T-cell subset that appears to be upregulated in Crohn's disease. The purpose of this study was to examine the expression of IRF-1 in the nuclei of lamina propria mononuclear cells in situ in colonoscopic biopsy specimens from pediatric patients with Crohn's disease, in patients with ulcerative colitis, and in control patients with no histopathologic abnormalities. Methods Archival paraffin-embedded tissue sections were obtained from 25 pediatric patients with Crohn's disease, 6 patients with ulcerative colitis, and 12 control patients who had undergone colonoscopy. Tissue sections were stained with polyclonal rabbit anti-human antisera to IRF-1 and horseradish-peroxidase-conjugated, biotinylated, goat anti-rabbit secondary antibody. Slides were scored and scores compared among patient groups using analysis of variance. Results Patients with Crohn's disease had significantly higher IRF-1 scores (95% confidence interval [CI], 1.70-2.04) than patients with ulcerative colitis (95% CI, 0.92-1.23) or control subjects (95% CI, 1.11-1.52). Conclusions Increased expression of IRF-1 in lamina propria mononuclear cells from patients with Crohn's disease may be relevant to the pathogenesis of Crohn's disease. |
| Databáze: | OpenAIRE |
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