Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic
| Autor: | Eva Juranová, Vera Ocenaskova, Katerina Sovova, Petra Vašíčková, Hana Mlejnková, Lucie Jašíková |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
Coronavirus disease 2019 (COVID-19) Health Toxicology and Mutagenesis Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses Population Pneumonia Viral coronavirus lcsh:Medicine virus 010501 environmental sciences Biology Wastewater Real-Time Polymerase Chain Reaction 01 natural sciences Virus epidemic 03 medical and health sciences Betacoronavirus Feces Humans education skin and connective tissue diseases Pandemics 030304 developmental biology 0105 earth and related environmental sciences Czech Republic 0303 health sciences education.field_of_study SARS-CoV-2 Communication lcsh:R fungi RT-qPCR Public Health Environmental and Occupational Health RNA COVID-19 respiratory tract diseases wastewater-based epidemiology Reverse transcription polymerase chain reaction body regions RNA Viral Sewage treatment Coronavirus Infections early warning system |
| Zdroj: | International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 17, Iss 5508, p 5508 (2020) |
| ISSN: | 1660-4601 1661-7827 |
| Popis: | The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population. |
| Databáze: | OpenAIRE |
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