Pathophysiology of middle ear epithelium: a new role for prostaglandin E2
| Autor: | Alain Grodet, Pen-Tung Yen, Philippe Herman, Roland Cassingena, Tzong-Yang Tu, Patrice Tran Ba Huy, Alain Loiseau, Gerard Friedlander |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty Mucociliary clearance Ear Middle Models Biological Dinoprostone Epithelium Cell Line otorhinolaryngologic diseases medicine Cyclic AMP Animals Prostaglandin E2 Cells Cultured Ion Transport business.industry Otitis Media with Effusion Sodium Fluid transport Cell Transformation Viral Mucus Electric Stimulation Cell biology Electrophysiology medicine.anatomical_structure Otitis Otorhinolaryngology Mucociliary Clearance Chronic Disease Middle ear medicine.symptom business Gerbillinae Intracellular medicine.drug |
| Zdroj: | American journal of otolaryngology. 15(4) |
| ISSN: | 0196-0709 |
| Popis: | Introduction: Otitis media with effusion is a disease of the middle ear epithelium resulting from a decreased sol layer as well as increased mucus secretion and plasma-derived protein transudation, which causes mucus plugging. Because the epithelium keeps the middle ear cavities fluid-free and air-filled, we investigated its fluid transport capacities, which may be involved in both efficiency of the mucociliary clearance and drying-out of the posterior ear cavities (Yen PT et al: Acta Otolaryngol (Stockh) 113, 1993). We have established the absorptive capacity of middle ear epithelial cells in primary culture (Herman P, et al: Am J Physiol 262, 1992). However, the paucity of cells obtained by enzymatic digestion led us to develop a new model for further investigation of middle ear epithelial cell. Methods: We established a middle ear cell line (MESV) using simian virus 40 (SV40) infection of middle ear epithelial cells from the Mongolian gerbil. Results: Investigation of the transport processes using the short-circuit current technique showed that MESV cells retain most characteristics of the original middle ear epithelial cells. Transepithelial sodium transport from the apical to the basal side was responsible for the transepithelial lumen-negative potential difference. Conclusion: The presence of high concentrations of prostaglandin E2 in the middle ear effusions has been documented. This work investigates the effect of prostglandin E2 on the rate of transepithelial ion transport of MESV cells. Prostaglandin E2 increased the rate of electrogenic sodium transport by means of increase in the intracellular cyclic adenosine monophosphate (cAMP) content. Such a modulation of sodium transport in the course of otitis media could be responsible for the reduced periciliary sol layer that impairs the mucociliary clearance. |
| Databáze: | OpenAIRE |
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