Role of beta-D-galactofuranose in Leishmania major macrophage invasion
| Autor: | Ameria K. Tanaka, Marcos S. Toledo, Helio K. Takahashi, Erika Suzuki, Anita H. Straus |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.drug_class
Immunology Antibodies Protozoan Leishmaniasis Cutaneous Biology Monoclonal antibody Phosphatidylinositols Microbiology Immunoglobulin Fab Fragments Mice Glycolipid medicine Macrophage Animals Leishmania major Amastigote Fluorescent Antibody Technique Indirect Chromatography High Pressure Liquid Infectivity Mice Inbred BALB C Kinetoplastida Antibodies Monoclonal Galactose biology.organism_classification Leishmania Infectious Diseases Macrophages Peritoneal Parasitology Chromatography Thin Layer Glycolipids Fungal and Parasitic Infections |
| Zdroj: | Infection and immunity. 70(12) |
| ISSN: | 0019-9567 |
| Popis: | The role of glycosylinositol phospholipid 1 (GIPL-1) ofLeishmania(Leishmania)majorin the interaction of promastigotes and amastigotes with macrophages was analyzed. Monoclonal antibody MEST-1, which recognizes glycolipids containing terminal galactofuranose (Galf) residues (E. Suzuki, M. S. Toledo, H. K. Takahashi, and A. H. Straus, Glycobiology7:463-468, 1997), was used to detect GIPL-1 inLeishmaniaby indirect immunofluorescence and to analyze its role in macrophage infectivity.L. majorpromastigotes showed intense fluorescence with MEST-1, and GIPL-1 was detected in both amastigote and promastigote forms by high-performance thin-layer chromatography immunostaining by using MEST-1. Delipidation ofL. majorpromastigotes with isopropanol-hexane-water eliminated the MEST-1 reactivity, confirming that only GIPL-1 is recognized in either amastigotes or promastigotes of this species. The biological role of GIPL-1 in the ability ofL. majorto invade macrophages was studied by using either Fab fragments of MEST-1 or methylglycosides. Preincubation of parasites with Fab fragments reduced macrophage infectivity in about 80% of the promastigotes and 30% of the amastigotes. Preincubation of peritoneal macrophages withp-nitrophenyl-β-galactofuranoside (10 mM) led to significant (∼80%) inhibition of promastigote infectivity. These data suggest that a putative new receptor recognizing β-d-Galf is associated withL. majormacrophage infectivity and that GIPL-1 containing a terminal Galf residue is involved in theL. major-macrophage interaction. |
| Databáze: | OpenAIRE |
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