Paclitaxel incorporated exosomes derived from glioblastoma cells: comparative study of two loading techniques
| Autor: | Soodeh Salarpour, Marzie Esmaeeli, Hamid Forootanfar, Meysam Ahmadi-Zeidabadi, Abbas Pardakhty, Mostafa Pournamdari |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Paclitaxel
Cell Survival Cell 02 engineering and technology Exosomes Exosome 03 medical and health sciences chemistry.chemical_compound Cell Line Tumor Humans Medicine Viability assay Particle Size Cytotoxicity Cell Proliferation 030304 developmental biology Drug Carriers 0303 health sciences Brain Neoplasms business.industry Building and Construction 021001 nanoscience & nanotechnology Antineoplastic Agents Phytogenic Microvesicles Cell biology medicine.anatomical_structure chemistry Cell culture Drug delivery Glioblastoma 0210 nano-technology business Research Article |
| Zdroj: | Daru |
| ISSN: | 2008-2231 |
| DOI: | 10.1007/s40199-019-00280-5 |
| Popis: | BACKGROUND: Exosomes are natural nanoparticles that are involved in intercellular communication via transferring molecular information between cells. Recently, exosomes have been considered for exploitation as novel drug delivery systems due to their specific properties for carrying specific molecules and surface proteins. METHODS: In this study, U-87 cell derived exosomes have been investigated for delivery of a potent chemotherapeutic agent, paclitaxel (PTX). Two methods of loading were utilized to incorporate PTX in exosomes and the exosomes pharmaceutical and cytotoxic characterizations were determined. RESULTS: The drug loaded and empty exosomes were found to have particle size of 50–100 nm and zeta potential of ≈ − 20 mV. Loading capacity of 7.4 ng and 9.2 ng PTX into 1 μg of exosome total protein were also measured for incubation and sonication methods, respectively. Incorporation of PTX into exosomes significantly increased its cytotoxicity against U-87 cell line (59.92% cell viability) while it was found that the empty exosomes exhibited cell viability of 91.98%. CONCLUSIONS: Loading method could affect the loading capacity of exosomes and their encapsulated chemotherapeutic molecule showed higher cytotoxicity into exosomes. These results promise exosomes as appropriate drug delivery system for glioblastoma multiform (GBM) treatment. |
| Databáze: | OpenAIRE |
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