Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression
| Autor: | Michael M. Neff, Courtney F. Pierce, Reuben Tayengwa, Pushpa Sharma Koirala, Breanna E. Werner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Flowers Plant Science AT-hook 01 natural sciences Flowering 03 medical and health sciences FT Gene Expression Regulation Plant lcsh:Botany Gene family Peptide sequence Gene Genetics biology Arabidopsis Proteins Intron Wild type food and beverages Plants Genetically Modified biology.organism_classification Phenotype lcsh:QK1-989 030104 developmental biology AHL AHL20 Research Article 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology, Vol 20, Iss 1, Pp 1-13 (2020) BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | BackgroundThe 29-member ArabidopsisAHLgene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains.AHLgene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored.ResultsTransgenic plants overexpressingAtAHL20flowered later than the wild type under both short and long days. Transcript accumulation analyses showed that35S:AtAHL20plants contained reducedFT, TSF, AGL8andSPL3mRNA levels. Similarly, overexpression ofAtAHL20’sorthologue inCamelina sativa,Arabidopsis’ closely relatedBrassicaceaefamily member species, conferred a late-flowering phenotype via suppression ofCsFTexpression. However, overexpression of an aberrantAtAHL20gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL19, AtAHL22 and AtAHL29.ConclusionWe showed via gain-of-function analysis thatAtAHL20is a negative regulator ofFTexpression, as well as other downstream flowering time regulating genes. A similar outcome inCamelina sativatransgenic plants overexpressingCsAHL20suggest that this is a conserved function. Our results demonstrate thatAtAHL20acts as a photoperiod-independent negative regulator of transition to flowering. |
| Databáze: | OpenAIRE |
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