Identification of tumor-initiating cells derived from two canine rhabdomyosarcoma cell lines
| Autor: | Toshiyuki Ishiwata, Eri Onozawa, T. E. Kishimoto, Makoto Ujike, Kimimasa Takahashi, Daigo Azakami, Shoko Yashima, Rei Nakahira, Masaki Michishita, Kazuhiko Ochiai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Vincristine
genetic structures 040301 veterinary sciences canine Antineoplastic Agents Tumor initiation tumor-initiating cells medicine.disease_cause 0403 veterinary science Mice 03 medical and health sciences Dogs 0302 clinical medicine Cancer stem cell Cell Line Tumor Rhabdomyosarcoma Pathology medicine Animals Dog Diseases Mice Inbred BALB C Muscle Neoplasms drug resistance Full Paper General Veterinary Chemistry 04 agricultural and veterinary sciences equipment and supplies medicine.disease In vitro body regions Doxorubicin Drug Resistance Neoplasm Cell culture 030220 oncology & carcinogenesis Canine Rhabdomyosarcoma Neoplastic Stem Cells Cancer research Female sphere sense organs Mitoxantrone Carcinogenesis Neoplasm Transplantation medicine.drug |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.16-0412 |
| Popis: | Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres derived from canine rhabdomyosarcoma cells have stem cell-like properties is unclear. We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres in vitro and in vivo. Sphere-forming cells were more resistant to vincristine, mitoxantrone and doxorubicin than adherent cells. Xenograft transplantation demonstrated that 1 × 103 sphere-forming cells derived from CMS-C were sufficient for tumor formation. The sphere assay showed that the sphere-forming cells were present in these tumors. These results suggest that the spheres derived from canine rhabdomyosarcoma cells may possess characteristics of TICs. This study provides the foundation for elucidating the contribution of TICs to rhabdomyosarcoma tumorigenesis. |
| Databáze: | OpenAIRE |
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