A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
| Autor: | Dany Liu, Maria Sole Sica, Jiwei Mao, Lucy Fang-I Chao, Verena Siewers |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Other Electrical Engineering
Electronic Engineering Information Engineering dynamic pathway control Other Engineering and Technologies not elsewhere specified flavonoids Biomedical Engineering transcriptional regulation transcription repressor General Medicine Control Engineering yeast Biochemistry Genetics and Molecular Biology (miscellaneous) |
| Zdroj: | ACS Synthetic Biology vol.In Press(2022) |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.2c00111 |
| Popis: | In vivo biosensors that can convert metabolite concentrations into measurable output signals are valuable tools for high-throughput screening and dynamic pathway control in the field of metabolic engineering. Here, we present a novel biosensor in Saccharomyces cerevisiae that is responsive to p-coumaroyl-CoA, a central precursor of many flavonoids. The sensor is based on the transcriptional repressor CouR from Rhodopseudomonas palustris and was applied in combination with a previously developed malonyl-CoA biosensor for dual regulation of p-coumaroyl-CoA synthesis within the naringenin production pathway. Using this approach, we obtained a naringenin titer of 47.3 mg/L upon external precursor feeding, representing a 15-fold increase over the nonregulated system. |
| Databáze: | OpenAIRE |
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