Effect of dDAVP on basolateral cell surface water permeability in the outer medullary collecting duct
| Autor: | Ivanova Ln, Khodus Gr, G. S. Baturina, Viatcheslav Nesterov, E. I. Solenov |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Osmosis
Vasopressin Cell Membrane Permeability Cell Cell Culture Techniques Biophysics Sensitivity and Specificity Cell membrane Osmotic Pressure medicine Animals Deamino Arginine Vasopressin Kidney Tubules Collecting Rats Wistar Cells Cultured Cell Size Osmole Water transport Chromatography Chemistry Reproducibility of Results Water Equipment Design General Medicine Apical membrane Epithelium Rats Equipment Failure Analysis medicine.anatomical_structure Tonicity |
| Zdroj: | European Biophysics Journal. 32:614-619 |
| ISSN: | 1432-1017 0175-7571 |
| DOI: | 10.1007/s00249-003-0308-9 |
| Popis: | We report a novel approach for assessing the volume of living cells which allows quantitative, high-resolution characterization of dynamic changes in cell volume while retaining the cell functionality. The aim of this study was to evaluate the short-term effect of vasopressin on basolateral cell surface water permeability in the outer medullary collecting duct (OMCD). The permeability of the basolateral cell membrane was determined in the tubules where the apical membrane was blocked with oil injected into the lumen. The apparent coefficient of water permeability (Pf) was evaluated by measuring the cell swelling after the step from hypertonic to isotonic medium (600 mosm to 300 mosm). Desmopressin (dDAVP) induced an increase of the basolateral Pf from 113.7+/-8.5 microm/s in control cells to 186.6+/-11.4 mum/s in micro-dissected fragments of the OMCD incubated in vitro (10(-7) M dDAVP, 30 min at 37 degrees C) (P |
| Databáze: | OpenAIRE |
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