A modified compression model of spinal cord injury in rats: functional assessment and the expression of nitric oxide synthases
| Autor: | Yu-Feng Su, Shinn-Cherng Chen, Tai-Hsin Tsai, Chih-Lung Lin, Aij-Lie Kwan, Shu-Chuan Wu, Shiuh-Lin Hwang, Kung-Shing Lee |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Catheters Nitric Oxide Synthase Type III Gene Expression Nitric Oxide Synthase Type II Severity of Illness Index Nitric oxide Rats Sprague-Dawley Random Allocation chemistry.chemical_compound Text mining Compression (functional analysis) medicine Animals RNA Messenger Spinal cord injury Spinal Cord Injuries Lumbar Vertebrae business.industry Laminectomy General Medicine medicine.disease Disease Models Animal Spinal Cord Neurology chemistry Anesthesia Original Article Neurology (clinical) business Locomotion |
| Zdroj: | Spinal Cord |
| ISSN: | 1476-5624 1362-4393 |
| DOI: | 10.1038/sc.2014.245 |
| Popis: | Study design: Experimental study. Objectives: To investigate a modified compression model of spinal cord injury (SCI) in adult rats by using a room-air- inflated Fogarty balloon catheter. Setting: Kaohsiung, Taiwan. Methods: The rats were divided into injury, sham-operated and control groups. A 2-French Fogarty catheter was passed from the lumbar spine (L3–L4) epidurally, with a mini-laminectomy under the microscope, to the level of thoracic spine (T6–T7). The actual site of the catheter tip was confirmed with X-ray. The balloon of Fogarty catheter then was inflated with room air, 0.2 ml, for 10 min. Mini-laminectomy was performed without inserting the catheter in the sham-operated group. Quantitative neurological outcomes were evaluated with the Basso, Beattie and Bresnahan (BBB) locomotor rating scale daily. The gene expression of nitric oxide synthases (NOSs) of the spinal cord was investigated at the end of the functional assessment. Results: The mean BBB locomotor scores were 10±1.85 and 10±1.85, respectively, on days 1 and 3 in the injury group, and 21 and 20.29±0.69, respectively, in the sham-operated group. There was a significantly increased gene expression of inducible NOS in the SCI group compared with the sham-operated group and control group. Endothelial NOS gene expression was not significantly different among the groups. Conclusion: The functional and molecular assessments show that this modified balloon-compression technique is a reproducible, simple and inexpensive model of SCI in rats. |
| Databáze: | OpenAIRE |
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