miR‐582‐5p serves as an antioncogenic biomarker in intermediate risk AML with normal cytogenetics and could inhibit proliferation and induce apoptosis of leukemia cells
| Autor: | Peihua Zhang, Fang-Xia Wang, Xiaman Wang, Ju Bai, Yuandong Feng, Aili He, Hongli Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Cellular differentiation Apoptosis Biology Young Adult 03 medical and health sciences 0302 clinical medicine Bone Marrow Cell Line Tumor hemic and lymphatic diseases microRNA Biomarkers Tumor medicine Humans Cell Proliferation Cell growth Cytogenetics Myeloid leukemia Cell Biology General Medicine Middle Aged Prognosis medicine.disease Leukemia Myeloid Acute MicroRNAs Cellular component organization Leukemia 030104 developmental biology Case-Control Studies 030220 oncology & carcinogenesis Cancer research Biomarker (medicine) Female |
| Zdroj: | Cell Biology International. 44:2021-2030 |
| ISSN: | 1095-8355 1065-6995 |
| Popis: | Numerous studies confirmed that aberrant microRNA (miRNA) expression contributes to cancer development and progression. We carried out this study to explore the expression profile of miRNAs in intermediate risk acute myeloid leukemia (AML) and locate certain miRNAs as biomarkers. We profiled differentially expressed miRNAs by performing miRNA sequencing analysis in the patients' samples. Bioinformatic analysis showed the most significantly expressed genes mostly involved in cellular component organization, cell differentiation, and cell development. Reverse-transcription polymerase chain reaction validated the expression of miR-582-5p in different groups of AML samples. It was confirmed that miR-582-5p was downregulated in newly diagnosed AML and relapse/refractory AML compared with CR AML or controls. Among intermediate risk AML patients with normal cytogenetics, a lower level of miR-582-5p is correlated with an unfavorable outcome, and a shorter overall survival. Gain- and loss-of-function experiments revealed that miR-582-5p could inhibit proliferation, suppress migration, and invasion ability and induce apoptosis of leukemia cells. Furthermore, overexpression of miR-582-5p can increase sensitivity of cells to Ara-C. In conclusion, miR-582-5p can serve as an antioncogenic biomarker in intermediate risk AML with normal cytogenetics for risk classification and outcome prediction. These results showed a novel role for miR-582-5p in predicting the prognosis and promoting the tumor growth of AML. |
| Databáze: | OpenAIRE |
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