Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge
| Autor: | Taiana Pereira Da Costa, Peter K. Kaiser, Mark P. Stevens, Andreas Alber, Cosmin Chintoan-Uta, Karen Bryson, Lonneke Vervelde |
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| Rok vydání: | 2019 |
| Předmět: |
Male
colibacillosis Systemic disease animal structures 040301 veterinary sciences chicken Dose-Response Relationship Immunologic Dose dependence heterophil Antibodies Heterophile macrophage Biology Microbiology 0403 veterinary science resistance Food Animals Pathogenic Escherichia coli Escherichia coli medicine Animals avian pathogenic E. Coli Respiratory system innate immunity Escherichia coli Infections Poultry Diseases Disease Resistance Air Sacs General Immunology and Microbiology Macrophages 0402 animal and dairy science 04 agricultural and veterinary sciences medicine.disease biology.organism_classification 040201 dairy & animal science Bacterial Load Immunity Innate Specific Pathogen-Free Organisms inbred lines Female Animal Science and Zoology Chickens Animals Inbred Strains |
| Zdroj: | Alber, A, Costa, T, Chintoan-Uta, C, Bryson, K J, Kaiser, P, Stevens, M P & Vervelde, L 2018, ' Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge ', Avian Pathology, pp. 1-33 . https://doi.org/10.1080/03079457.2018.1562154 |
| DOI: | 10.6084/m9.figshare.7585820.v1 |
| Popis: | Avian pathogenic E. coli (APEC) cause severe respiratory and systemic disease. To address the genetic and immunological basis of resistance, inbred chicken lines were used to establish a model of differential resistance to APEC, using strain O1 of serotype O1:K1:H7. Inbred lines 72, 15I and C.B12 and the outbred line Novogen Brown were inoculated via the airsac with a high dose (107 colony-forming units, CFU) or low dose (105 CFU) of APEC O1. Clinical signs, colibacillosis lesion score and bacterial colonization of tissues after high dose challenge were significantly higher in line 15I and C.B12 birds. The majority of the 15I and C.B12 birds succumbed to the infection by 14 h post-infection, whilst none of the line 72 and the Novogen Brown birds developed clinical signs. No difference was observed after low dose challenge. In a repeat study, inbred lines 72 and 15I were inoculated with low, intermediate or high doses of APEC O1 ranging from 105 to 107 CFU. The colonization of lung was highest in line 15I after high dose challenge and birds developed clinical signs; however, colonization of blood and spleen, clinical signs and lesion score were not different between lines. No difference was observed after intermediate or low dose challenge. Ex vivo, the phagocytic and bactericidal activity of lung leukocytes from line 72 and 15I birds did not differ. Our data suggest that although differential resistance of inbred lines 72, 15I and C.B12 to APEC O1 challenge is apparent, it is dependent on the infectious dose. Research HighlightsLines 15I and C.B12 are more susceptible than line 72 to a high dose of APEC O1.Differential resistance is dose-dependent in lines 15I and 72.Phagocytic and bactericidal activity is similar and dose independent. Lines 15I and C.B12 are more susceptible than line 72 to a high dose of APEC O1. Differential resistance is dose-dependent in lines 15I and 72. Phagocytic and bactericidal activity is similar and dose independent. |
| Databáze: | OpenAIRE |
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