Targeted polymeric nanoparticles for drug delivery to hypoxic, triple-negative breast tumors
| Autor: | Jagadish Loganathan, Venkatachalem Sathish, Nimesha De Fonseka, Daniel M Tuvin, Babak Mamnoon, Sanku Mallik, Jamie Froberg, Matthew Confeld, Yongki Choi, Li Feng |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Polymers Biomedical Engineering Mice Nude Antineoplastic Agents Triple Negative Breast Neoplasms Article Biomaterials Mice Drug Delivery Systems Cell Line Tumor medicine Animals Humans Doxorubicin Molecular Structure Chemistry Biochemistry (medical) General Chemistry Xenograft Model Antitumor Assays Neuropilin-1 Oxygen tension Gene Expression Regulation Neoplastic Oxygen Drug Liberation Targeted drug delivery Transcytosis Polymersome Drug delivery Cancer research Nanoparticles Female Nanocarriers Drug carrier medicine.drug |
| Zdroj: | ACS Appl Bio Mater |
| Popis: | High recurrence and metastasis to vital organs are the major characteristics of triple-negative breast cancer (TNBC). Low vascular oxygen tension promotes resistance to chemo- and radiation therapy. Neuropilin-1 (NRP-1) receptor is highly expressed on TNBC cells. The tumor-penetrating iRGD peptide interacts with the NRP-1 receptor, triggers endocytosis and transcytosis, and facilitates penetration. Herein, we synthesized a hypoxia-responsive diblock PLA-diazobenzene-PEG copolymer and prepared self-assembled hypoxia-responsive polymersomes (Ps) in an aqueous buffer. The iRGD peptide was incorporated into the polymersome structure to make hypoxia-responsive iRGD-conjugated polymersomes (iPs). Doxorubicin (DOX) was encapsulated in the polymersomes to prepare both targeted and non-targeted hypoxia-responsive polymersomes (DOX-iPs and DOX-Ps, respectively). The polymeric nanoparticles released less than 30% of their encapsulated DOX within 12 hours under normoxic conditions (21% oxygen), whereas under hypoxia (2% Oxygen), doxorubicin release remarkably increased to over 95%. The targeted polymersomes significantly decreased TNBC cells' viability in monolayer and spheroid cultures under hypoxia compared to normoxia. Animal studies displayed that targeted polymersomes significantly diminished tumor growth in xenograft nude mice. Overall, the targeted polymersomes exhibited potent anti-tumor activity in monolayer, spheroid, and animal models of TNBC. With further developments, the targeted nanocarriers discussed here might have the translational potential as drug carriers for the treatment of TNBC. |
| Databáze: | OpenAIRE |
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