scRNA-seq Profiling of Human Testes Reveals the Presence of the ACE2 Receptor, A Target for SARS-CoV-2 Infection in Spermatogonia, Leydig and Sertoli Cells
| Autor: | Zhengpin Wang, Xiaojiang Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine viruses Pneumonia Viral ACE2 Peptidyl-Dipeptidase A Biology Virus Replication medicine.disease_cause Article Male gamete generation Transcriptome 03 medical and health sciences 0302 clinical medicine RNA Small Cytoplasmic Testis scRNA-seq medicine Humans Receptor Pandemics Gene lcsh:QH301-705.5 Coronavirus Regulation of gene expression 030219 obstetrics & reproductive medicine SARS-CoV-2 Gene Expression Profiling COVID-19 General Medicine Sertoli cell infection Cell biology spermatogonia Gene expression profiling 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation lcsh:Biology (General) Receptors Virus Angiotensin-Converting Enzyme 2 Coronavirus Infections hormones hormone substitutes and hormone antagonists |
| Zdroj: | Cells Volume 9 Issue 4 Cells, Vol 9, Iss 920, p 920 (2020) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells9040920 |
| Popis: | In December 2019, a novel coronavirus (SARS-CoV-2) was identified in COVID-19 patients in Wuhan, Hubei Province, China. SARS-CoV-2 shares both high sequence similarity and the use of the same cell entry receptor, angiotensin-converting enzyme 2 (ACE2), with severe acute respiratory syndrome coronavirus (SARS-CoV). Several studies have provided bioinformatic evidence of potential routes of SARS-CoV-2 infection in respiratory, cardiovascular, digestive and urinary systems. However, whether the reproductive system is a potential target of SARS-CoV-2 infection has not yet been determined. Here, we investigate the expression pattern of ACE2 in adult human testes at the level of single-cell transcriptomes. The results indicate that ACE2 is predominantly enriched in spermatogonia and Leydig and Sertoli cells. Gene Set Enrichment Analysis (GSEA) indicates that Gene Ontology (GO) categories associated with viral reproduction and transmission are highly enriched in ACE2-positive spermatogonia, while male gamete generation related terms are downregulated. Cell&ndash cell junction and immunity-related GO terms are increased in ACE2-positive Leydig and Sertoli cells, but mitochondria and reproduction-related GO terms are decreased. These findings provide evidence that the human testis is a potential target of SARS-CoV-2 infection, which may have significant impact on our understanding of the pathophysiology of this rapidly spreading disease. |
| Databáze: | OpenAIRE |
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